|IP||0.2-1 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Mouse CD86 was immunoprecipitated using:
Lane A:0.5 mg Raji Whole Cell Lysate0.5 µL anti- Mouse CD86 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti- Mouse CD86 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 42 kDa
Observed band size: 42 kDa
Anti-CD86 rabbit polyclonal antibody at 1:500 dilution
Lane A: Jurkat Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:70 kDa
Observed band size:100 kDa
(We are unsure as to the identity of these extra bands.)
CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.
Immune Checkpoint Detection: Antibodies Immune Checkpoint Detection: ELISA Antibodies Immune Checkpoint Detection: IP Antibodies Immune Checkpoint Detection: FCM Antibodies Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets Co-inhibitory Immune Checkpoint Targets
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD86 Antibody (PE)||Mouse||Monoclonal||FCM||80025-MM10-P|
|Anti-CD86 Antibody (PerCP)||Mouse||Monoclonal||FCM||10699-MM06-C|
|Anti-CD86 Antibody (FITC)||Mouse||Monoclonal||FCM||10699-MM06-F|
|Anti-CD86 Antibody (APC)||Mouse||Monoclonal||FCM||10699-MM06-A|
|Anti-CD86 Antibody (PE)||Mouse||Monoclonal||FCM||10699-MM06-P|