Anti-CD86 Antibody

Cat: 50068-RP02
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All CD86 Reagents
Anti-CD86 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD86 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD86
Immunogen
Recombinant Mouse CD86 protein (Catalog#50068-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD86 extracellular domain (rM CD86; Catalog#50068-M08H; NP_062261.3; Met 1-Glu 245). CD86 specific IgG was purified by mouse CD86 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD86 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD86 Antibody (Rabbit Polyclonal antibody) Images
Mouse CD86/B7-2 Immunoprecipitation(IP) 6086

Mouse CD86 was immunoprecipitated using:

Lane A:0.5 mg Raji Whole Cell Lysate

0.5 µL anti- Mouse CD86 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti- Mouse CD86 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 42 kDa

Observed band size: 42 kDa

Mouse CD86/B7-2 Western blot (WB) 13641

Anti-CD86 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:70 kDa

Observed band size:100 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD86 Antibody Alternative Names
Anti-B7 Antibody;Anti-B7-2 Antibody;Anti-B7.2 Antibody;Anti-B70 Antibody;Anti-Cd28l2 Antibody;Anti-CLS1 Antibody;Anti-ETC-1 Antibody;Anti-Ly-58 Antibody;Anti-Ly58 Antibody;Anti-MB7 Antibody;Anti-MB7-2 Antibody;Anti-TS/A-2 Antibody
CD86 Background Information

CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

Immune Checkpoint
Immune Checkpoint Detection: Antibodies   Immune Checkpoint Detection: ELISA Antibodies   Immune Checkpoint Detection: IP Antibodies   Immune Checkpoint Detection: FCM Antibodies   Immune Checkpoint Detection: WB Antibodies
Immune Checkpoint Proteins
Immune Checkpoint Targets   Co-inhibitory Immune Checkpoint Targets

Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Full Name
CD86 antigen
References
  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 90270-RP01
    Anti-CD86 Antibody Rabbit Monoclonal ELISA 90270-R021
    Anti-CD86 Antibody Mouse Monoclonal ELISA(Cap) 80025-MM12
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 80025-T16
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 80025-RP02
    Anti-CD86 Antibody (PE) Mouse Monoclonal FCM 80025-MM10-P
    Anti-CD86 Antibody Mouse Monoclonal ELISA(Det),FCM 80025-MM10
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 80025-RP01
    Anti-CD86 Antibody Mouse Monoclonal ELISA 80025-MM04
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 50068-RP01
    Anti-CD86 Antibody Rabbit Monoclonal ELISA 50068-R003
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 50068-T16
    Anti-CD86 Antibody (PerCP) Mouse Monoclonal FCM 10699-MM06-C
    Anti-CD86 Antibody (FITC) Mouse Monoclonal FCM 10699-MM06-F
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 10699-RP02
    Anti-CD86 Antibody Rabbit Monoclonal ELISA,ELISA(Det) 10699-R118
    Anti-CD86 Antibody Rabbit Monoclonal ELISA(Cap) 10699-R103
    Anti-CD86 Antibody (APC) Mouse Monoclonal FCM 10699-MM06-A
    Anti-CD86 Antibody Rabbit Monoclonal IHC-P 10699-R269
    Anti-CD86 Antibody Mouse Monoclonal ELISA 10699-MM02
    Anti-CD86 Antibody (PE) Mouse Monoclonal FCM 10699-MM06-P
    Anti-CD86 Antibody Mouse Monoclonal FCM 10699-MM06
    Anti-CD86 Antibody Rabbit Polyclonal ELISA 10699-RP01
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