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CD86 / B7-2 / B70 Antibody, Rabbit PAb

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Human B7-2/CD86 Antibody Product Information
Immunogen:Recombinant Human CD86 protein (Catalog#10699-H08H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CD86 / B7-2 (rhCD86; Catalog#10699-H08H; NP_008820.2; Met 1-His 239). Total IgG was purified by Protein A affinity chromatography.
Human B7-2/CD86 Antibody Usage Guide
Specificity:Human CD86 / B7-2 / B70 / CD28LG2
Application:ELISA

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD86. The detection limit for Human CD86 is 0.00245 ng/well.

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Other B7-2/CD86 Antibody Products
Reactivity: Human  
Application: ELISA  
    10699-RP02-50
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    200 µg 
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    Reactivity: Human  
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      10699-MM02-50
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      Reactivity: Human  
      Application: ELISA  
        10699-R118-50
        10699-R118-100
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        Reactivity: Human  
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        • Slide 1
        10699-MM06-F-50
        10699-MM06-F-100
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        Reactivity: Human  
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        • Slide 1
        10699-MM06-P-50
        10699-MM06-P-100
        50 Tests 
        100 Tests 
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        Reactivity: Human  
        Application: FCM  
        • Slide 1
        10699-MM06-200
        10699-MM06-100
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        200 µg 
        100 µg 
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        • Slide 1
        10699-MM06-A-100
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        100 Tests 
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        Reactivity: Mouse  
        Application: ELISA  
          50068-RP01-100
          50068-RP01-200
          50068-RP01-400
          100 µg 
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          Reactivity: Mouse  
          Application: ELISA  WB  IP  
          • Slide 1
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          50068-RP02-50
          50068-RP02-100
          50068-RP02-200
          50 µg 
          100 µg 
          200 µg 
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          Reactivity: Mouse  
          Application: ELISA  
            50068-R003-50
            50068-R003-100
            50 µg 
            100 µg 
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            Reactivity: Rat  
            Application: ELISA  
              80025-RP02-50
              80025-RP02-100
              80025-RP02-200
              50 µg 
              100 µg 
              200 µg 
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              Reactivity: Rat  
              Application: ELISA  
                80025-MM04-50
                80025-MM04-100
                80025-MM04-200
                50 µg 
                100 µg 
                200 µg 
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                Reactivity: Rat  
                Application: ELISA  
                  80025-RP01-200
                  80025-RP01-100
                  80025-RP01-400
                  200 µg 
                  100 µg 
                   
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                  Reactivity: Cynomolgus  
                  Application: ELISA  
                    90270-R021-100
                    90270-R021-50
                    100 µg 
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                    CD86/B7-2 Background

                    CD86, also known as B-lymphocyte activation antigen B7-2 (referred to as B70), is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus induces the signal pathways which regulate T cell activation and tolerance, cytokine production, and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis, allergic pulmonary inflammation, arthritis, and antiviral responses, and thus is regarded as a promising candidate for immune therapy.

                    Human CD86/B7-2 References
                  • Chen YQ, et al. (2006) CD28/CTLA-4--CD80/CD86 and ICOS--B7RP-1 costimulatory pathway in bronchial asthma. Allergy. 61(1): 15-26.
                  • Rau FC, et al. (2009) B7-1/2 (CD80/CD86) direct signaling to B cells enhances IgG secretion. J Immunol. 183(12): 7661-71.
                  • Dai ZS, et al. (2009) Defective expression and modulation of B7-2/CD86 on B cells in B cell chronic lymphocytic leukemia. Int J Hematol. 89(5): 656-63.
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                    Catalog: 10699-RP01-100
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                    200 µg
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                    All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
                    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"