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CD84 ELISA Pair Set, Human ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK10100 |
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Human CD84 ELISA Pair Set
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Order or Inquire for CD84 ELISA Pair Set |
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High affinity Human CD84 ELISA Pair Set |
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Detection limit - 156.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Human CD84 ELISA Pair Set Related Products & Topics
Related Areas:
Immunology>>Adaptive Immunity>>Costimulation & Costimulatory Molecule>>CD84
Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD84
Immunology>>Cluster of Differentiation>>Monocyte/Macrophage CD Antigen>>Macrophage Markers / Monocyte Markers>>CD84
Immunology>>Cluster of Differentiation>>T Cell CD Antigen>>Helper T Cells>>CD84
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| CD84/SLAMF5 | Human | CD84/SLAMF5 Protein, Recombinant | 10100-H08H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Human CD84/SLAMF5 | WB, ELISA | Mouse Monoclonal Antibody | 10100-MM03 |
| Human CD84/SLAMF5 | WB, ELISA | Rabbit Monoclonal Antibody | 10100-R006 |
| Human CD84/SLAMF5 | WB, ELISA | Rabbit Polyclonal Antibody | 10100-RP01 |
| Human CD84/SLAMF5 | WB, ELISA | Rabbit Polyclonal Antibody (Antigen Affinity Purified) | 10100-RP02 |
Background
The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein ) and EAT-2 ( EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. Human CD84 is expressed as a single chain on dendritic cells, monocytes, macrophages, eosinophils, mast cells, granulocytes, and platelets. As a self-ligand, only the first N-terminal Ig V domain is requisite for the homotypic CD84-CD84 interaction, and it has been demonstrated that CD84 homophilic engagement enhances TCR-induced IFN-γ secretion and induces platelet stimulation.
