Anti-CD84 Antibody

Cat: 10100-R006
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All CD84 Reagents
Anti-CD84 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD84 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CD84
Immunogen
Recombinant Human CD84 protein (Catalog#10100-H08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD84 extracellular domain (rh CD84; Catalog#10100-H08H; Met 1-Gly 225; NP_003865.1).
Source
Monoclonal Rabbit IgG Clone #6
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD84 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.2-1 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD84 Antibody (Rabbit Monoclonal antibody) Images
Human CD84 Immunoprecipitation(IP) 6135

CD84 was immunoprecipitated using:

Lane A:0.5 mg Raw264.7 Whole Cell Lysate

0.5 µL anti-CD84 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD84 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 62 kDa

Observed band size: 62 kDa

Human CD84 Western blot (WB) 13117

Anti-CD84 rabbit monoclonal antibody at 1:500 dilution

Lane A: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:39 kDa

Observed band size:39 kDa

Anti-CD84 Antibody Alternative Names
Anti-hCD84 Antibody;Anti-LY9B Antibody;Anti-mCD84 Antibody;Anti-SLAMF5 Antibody
CD84 Background Information

The CD2 family receptors are type I transmembrane glycoproteins belonging to immunoglobulin (Ig) superfamily characterized by a membrane-proximal Ig constant 2 (C2) domain and a membrane-distal variable (V) domain that is responsible for ligand recognition. CD84, also known as LY9B and SLAMF5, is a homophilic member of the SLAM (signaling lymphocyte activation molecule) subfamily of the CD2 family. The SLAM family receptorsmediate signal transduction through the interaction of its ITSM (immunoreceptor tyrosine-based switch motifs) in the intracellular region and the SH2 domain of adaptor molecules SAP (SLAM-associated protein) and EAT-2 (EWS-activated transcript 2), and accordingly modulate both adaptive and innate immune responses. The CD84-CD84 interaction was independent of its cytoplasmic tail. Thus, CD84 is its own ligand and acts as a costimulatory molecule. CD84 is expressed on cells from almost all hematopoietic lineages and on CD34+ hematopoietic progenitor cells, suggesting that CD84 serves as a marker for committed hematopoietic progenitor cells.

Full Name
CD84 molecule
References
  • Martin M, et al. (2001) CD84 functions as a homophilic adhesion molecule and enhances IFN-gamma secretion: adhesion is mediated by Ig-like domain 1. J Immunol. 167(7): 3668-76.
  • Tangye SG, et al. (2002) CD84 is up-regulated on a major population of human memory B cells and recruits the SH2 domain containing proteins SAP and EAT-2. Eur J Immunol. 32(6): 1640-9.
  • Zaiss M, et al. (2003) CD84 expression on human hematopoietic progenitor cells. Exp Hematol. 31(9): 798-805.
  • Tangye SG, et al. (2003) Functional requirements for interactions between CD84 and Src homology 2 domain-containing proteins and their contribution to human T cell activation. J Immunol. 171(5): 2485-95.
  • Yan Q, et al. (2007) Structure of CD84 provides insight into SLAM family function. Proc Natl Acad Sci U S A. 104(25): 10583-8.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD84 Antibody Rabbit Polyclonal ELISA 50757-T16
    Anti-CD84 Antibody Rabbit Polyclonal ELISA 50757-RP01
    Anti-CD84 Antibody Mouse Monoclonal ELISA 10100-MM03
    Anti-CD84 Antibody Mouse Monoclonal ELISA(Det) 10100-MM05
    Anti-CD84 Antibody Rabbit Polyclonal ELISA 10100-RP02
    Anti-CD84 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 10100-R004
    Anti-CD84 Antibody Rabbit Polyclonal ELISA 10100-RP01
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