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Rat CD83 Gene cDNA Clone / ORF Clone

Catalog Vector (Price) Quantity In Stock Operation
RG80333-G





  YES

*  pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors.
    Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD83 cDNA Clone Datasheet

 

CD83 cDNA Clone Product Information

Gene Bank Ref.ID: NM_001108410.1

cDNA Size:

592

Gene Synonym:

Cd83

Source: Rat

CD83 cDNA Clone Description

cDNA Description:

ORF Clone of Rattus norvegicus Cd83 molecule DNA.

Vector: Cloning Vector: pGEM-T Vector

Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 276 C/T not causing the amino acid variation.

CD83 cDNA Clone Shipping

  1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
  2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD83 cDNA Clone Usage Protocol

  1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
  2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
  3. Transfer the tube to incubation at 95℃ for 15-30 min.
  4. At the end of the incubation pulse vortex, or gently tap the tube.
  5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD83 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTCGCAAG GCCTCCAGCT CCTGCTTCTA GGCTGCGCCT GCAGCCTGGC GCCCGCGTTG
61       GCGATGCGGG AGGTGACGGT GGCTTGCTCC GAAACGGCCG ACTTGCCGTG CACAGCGCCC
121      TGGGACCCGC AGCTCTCCTA CACAGTGTCC TGGGCCAAGG TCTCAGAGAG TGGCAACGAG
181      AGGCTGGAGC TCCCTGAGAG CAAGCAAAAC AGCTCCGTCG AGGCCCCCAA GAAAAGGCCT
241      TATTCCTTGA CAATCCAAAA CACTACGATC TGCAGTTGCT GGCACCTACA GGTGTGCCTT
301      GCAGGAGCTT GGAGGGCAAC GTAACTTCAG TGGCACAGTG GTTCTGAAGG TGACAGGATG
361      TCCCAAGGAA GCTACAGAGT CAACCTTCAG GAAGTACAGG GCGGAAGCTG TGTTGCTCTT
421      CTCTCTGGTC GTTTTCTACC TGACACTCAT CATTTTCACA TGTAAGTTTG CACGACTACA
481      GAGTATTTTT CCAGATATTT CTAAACCTGG TACAGAACAA GCTTTTCTTC CAGTCACCTC
541      CCCAAGCAAA CATTTGGGGC CAGTGACCCT TCCCAAGACA GAAACAGTAT GA

 

Quality Control:cDNA sequencing results is available upon request.

 

 

CD83 related areas, pathways, and other information