Mouse CD83 / HB15 Protein (His Tag)

Mouse CD83 Antigen / B-cell Activation Protein

Mouse CD83 Protein Product Information

• Synonym: MGC130312, MGC130313
• Protein Construction: A DNA sequence encoding the extracellular domain of mouse CD83 ( O88324 ) ( Met 1 - Arg 133 ) was expressed, with a C-terminal polyhistidine tag
• Source: Mouse
• Expression Host: Human Cells

Mouse CD83 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Met 22
• Molecular Mass: The secreted recombinant mouse CD83 comprises 123 amino acids and has a calculated molecular mass of 13.6 kDa. As a result of glycosylation, the apparent molecular mass of rm CD83 is approximately 25 - 35 kDa in SDS-PAGE under reducing conditions
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD83 protein

Mouse CD83 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Mouse CD83 Protein Description

Mouse CD83 antigen, also known as B-cell activation protein, Cell surface protein HB15 and CD83, is a single-pass type I membrane protein which contains one Ig-like V-type (immunoglobulin-like) domain. CD83 is a a conserved member of the immunoglobulin superfamily. CD83 is expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells. CD83 is a fascinating cell surface molecule with a unique pattern of expression that has multiple confirmed functions in regulating immune system development and function. CD83 represents an intriguing target for immunotherapy due to its preferential expression on mature dendritic cells (DCs), the most efficient of antigen presenting cells. On the protein level, two different isoforms of CD83 exist: a membrane-bound and a soluble form. The isoforms are generated by different subsets of cells, including DCs, T-cells and B-cells, and also differ in their biological function. While the membrane-bound CD83 is of immune stimulatory capacity, activates T-cells and is important for the generation of thymocytes, the soluble CD83 has the opposite effect and has an immune inhibitory capacity. Due to its immune inhibitory function. CD83 may play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation. CD83 has great potential for treatment of autoimmune diseases, for organ transplantations, and for immunotherapy , just to name a few examples. Moreover, some viruses prevent recognition by the host's immune system by specifically targeting CD83 surface expression.

References

1. Lechmann,M. et al., 2002, Trends Immunol. 23 (6):273-5.
2. Wolenski,M.  et al., 2003, Med Microbiol Immunol  192 (4):189-92.
3. Prechtel,A.T. et al., 2007, Arch Dermatol Res. 299 (2):59-69.
4. Doñate,C. et al., 2007, Fish Shellfish Immunol. 23 (4):877-85.
5. Prazma,C.M. et al., 2008, Immunol Lett. 115 (1):1-8.
6. Breloer,M. et al., 2008, Trends Immunol. 29 (4):186-94.