CD82 / KAI-1 Antibody (Antigen Affinity Purified)

CD82 / KAI-1 Antibody ( Antigen Affinity Purified ) Datasheet

CD82 / KAI-1 Antibody Product Information

• Immunogen: Recombinant Human CD82 / KAI-1 protein (Catalog#12275-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly 111-Leu 228). CD82 / KAI-1 specific IgG was purified by Human CD82 / KAI-1 affinity chromatography.

CD82 / KAI-1 Antibody Usage Guide

• Specificity: Human CD82 / KAI-1
• Cross reactivity: Reacts with
  

  Mouse, Rat

• Immunochemistry: IHC-P: 0.1-2 μg/mL

  

  Immunochemical staining of human CD82 in human endometrium with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelium in uterine-gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD82 in WB. Using a DAB detection system, the detection limit for Human CD82 is approximately 1 ng/lane under non-reducing conditions and 4 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD82. The detection limit for Human CD82 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD82 / KAI-1 Antibody Background

The transmembrane glycoprotein CD82, also known as Kangai (KAI)-1, is a member of the tetraspanin protein family and is a metastasis suppressor implicated in biological processes ranging from fusion, adhesion and migration to apoptosis and cell-morphology alterations. KAI1/CD82 is a candidate metastasis suppressor gene and has been indicated in the disease progression of certain solid tumours. KAI1 plays an important role in cell adhesion, invasion and migration of breast cancer cells, in vitro, and is a potential metastasis suppressor gene in breast cancer. The present study was designed to investigate the expression pattern of KAI-1 in the normal endometrium and uterine tumors and to correlate it with the expression of tumor suppressor protein p53. KAI-1 and p53 show inverse expression. The reduced KAI-1 expression may be the result of dysregulated p53 function and could be an important step in the endometrial carcinogenesis. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. Thus, CD82 may be a therapeutic approach for the treatment of a variety of metastatic cancers.

References

1. Tonoli H. et al., 2005, Trends Mol Med. 11 (12): 563-70.
2. Kim B. et al., 2010, Biochem Biophys Res Commun. 393 (1): 179-84.
3. Briese J. et al., 2008, Virchows Arch. 453 (1): 89-96.
4. Liu WM. et al., 2006, Cancer Lett. 240 (2): 183-94.