Anti-CD82 Antibody

Cat: 12275-RP02

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Anti-CD82 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD82 Antibody
Validated applications
Species reactivity
Reacts with: Human, Mouse, Rat
Human CD82
Recombinant Human CD82 / KAI-1 protein (Catalog#12275-H08H)
Produced in rabbits immunized with purified, recombinant Human CD82 / KAI-1 (rh CD82 / KAI-1; Catalog#12275-H08H; P27701-1; Gly 111-Leu 228). CD82 / KAI-1 specific IgG was purified by Human CD82 / KAI-1 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD82 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IHC-P 1:500-1:2000  
IP 1-4 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD82 Antibody (Rabbit Polyclonal antibody) Images
Human CD82/KAI-1 Immunohistochemistry(IHC) 4464
Immunochemical staining of human CD82 in human endometrium with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of epithelium in uterine-gland. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human CD82/KAI-1 Immunoprecipitation(IP) 8132

CD82 was immunoprecipitated using:

Lane A:0.5 mg A549 Whole Cell Lysate

Lane B:0.5 mg U87MG Whole Cell Lysate

2 µL anti-CD82 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD82 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 30 kDa

Observed band size: 36 kDa

Human CD82/KAI-1 Western blot (WB) 13482

Anti-CD82 rabbit polyclonal antibody at 1:500 dilution

Lane A: A549 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:30 kDa

Observed band size:32 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD82 Antibody Alternative Names
Anti-4F9 Antibody;Anti-C33 Antibody;Anti-GR15 Antibody;Anti-IA4 Antibody;Anti-KAI-1 Antibody;Anti-KAI1 Antibody;Anti-R2 Antibody;Anti-SAR2 Antibody;Anti-ST6 Antibody;Anti-TSPAN27 Antibody
CD82 Background Information

CD82, also known as KAI-1, structurally belongs to tetraspanin family while categorised as metastasis suppressor gene on functional grounds. KAI1/CD82 is localized on cell membrane and form interactions with other tetraspanins, integrins and chemokines which are respectively responsible for cell migration, adhesion and signalling. Downregulation of CD82 expression is associated with the advanced stages of many human cancers and correlates with the acquisition of metastatic potential. Recent studies suggest that complex mechanisms underlie CD82 loss of function, including altered transcriptional regulation, splice variant production and post-translational protein modifications, and indicate a central role for CD82 in controlling metastasis as a 'molecular facilitator'. The loss of KAI1/CD82 expression in invasive and metastatic cancers is due to a complex, epigenetic mechanism that probably involves transcription factors such as NFkappaB, p53, and beta-catenin. A loss of KAI1 expression is also associated with the advanced stages of many human malignancies and results in the acquisition of invasive and metastatic capabilities by tumour cells. Thus, KAI1/CD82 is regarded as a wide-spectrum tumor metastasis suppressor.

Full Name
CD82 molecule
  • Malik FA, et al. (2009) KAI-1/CD82, the molecule and clinical implication in cancer and cancer metastasis. Histol Histopathol. 24(4): 519-30.
  • Liu WM, et al. (2006) KAI1/CD82, a tumor metastasis suppressor. Cancer Lett. 240(2): 183-94.
  • Tonoli H, et al. (2006) CD82 metastasis suppressor gene: a potential target for new therapeutics? Trends Mol Med. 11(12): 563-70.
  • Jackson P, et al. (2005) KAI1 tetraspanin and metastasis suppressor. Int J Biochem Cell Biol. 37(3): 530-4.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD82 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 12275-R071
    Anti-CD82 Antibody Mouse Monoclonal IHC-P 12275-MM01
    Anti-CD82 Antibody Rabbit Polyclonal ELISA 12275-RP01
    Anti-CD82 Antibody Mouse Monoclonal IHC-P 12275-MM07
    Anti-CD82 Antibody Rabbit Monoclonal ELISA(Det) 12275-R072
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