Rat CD81 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
RG80331-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD81 cDNA Clone Datasheet

CD81 cDNA Clone Product Information

Gene Bank Ref.ID:	BC060583.1
cDNA Size:	711
Gene Synonym:	Tapa1; Cd81
Source:	Rat

CD81 cDNA Clone Description

cDNA Description:	ORF Clone of Rattus norvegicus Cd81 molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 12 A/G not causing the amino acid variation.

CD81 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD81 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD81 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGGGTGG AGGGCTGCAC CAAATGCATC AAATACCTGC TCTTCGTCTT CAATTTCGTC
61       TTCTGGCTGG CTGGAGGTGT GATCCTAGGT GTAGCTCTGT GGTTGCGCCA TGATCCACAG
121      ACCACCAGCC TTCTCTACCT GGAACTGGGA GACAAACCAG CACCTAGCAC CTTCTATGTG
181      GGCATCTACA TTCTCATTGC TGTGGGAGCT GTGATGATGT TTGTAGGCTT CCTGGGGTGC
241      TATGGGGCCA TCCAGGAGTC CCAGTGCCTG CTGGGGACGT TCTTCACTTG CCTTGTGATC
301      CTGTTTGCCT GTGAGGTAGC TGCGGGCATC TGGGGCTTTG TAAACAAAGA CCAGATCGCC
361      AAGGATGTGA AGCAGTTCTA CGACCAGGCC CTTCAGCAGG CTGTGATGGA TGATGATGCT
421      AACAATGCCA AGGCAGTGGT GAAGACCTTC CACGAGACGC TCAACTGTTG TGGCTCCAAT
481      ACGCTGACCA CACTGACCAC CACCGTGCTG AGGAACAGCC TGTGTCCCTC AAGCAGCAAC
541      TCATTCACTC AGCTTCTGAA GGAAGACTGC CATCAGAAAA TCGATGAGCT CTTTTCTGGG
601      AAGCTGTACC TTATTGGAAT TGCAGCCATT GTGGTAGCTG TCATTATGAT CTTCGAGATG
661      ATTCTGAGCA TGGTGCTGTG CTGTGGCATC CGGAACAGCT CCGTGTACTG A

Quality Control:cDNA sequencing results is available upon request.