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CD80 / B7-1 Antibody Datasheet
|Order or Inquire for CD80 / B7-1 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.039 ng/well in ELISA|
CD80 / B7-1 Antibody Product Information
Recombinant Mouse CD80 / B7-1 protein (Catalog#50446-M08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Mouse CD80 / B7-1 (rM CD80 / B7-1; Catalog#50446-M08H; Q00609-1; Met 1-Lys 245). Total IgG was purified by Protein A affinity chromatography.
CD80 / B7-1 Antibody Usage Guide
Mouse CD80 / B7-1
|Western blot :||This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD80 in WB.|
|Direct ELISA :||This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD80. The detection limit for Human CD80 is approximately 0.039 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD80 / B7-1 Antibody Related Products & Topics
|Molecule||Species||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|CD80/B7-1||Human||CD80/B7-1/Fc Protein, Recombinant||10698-H03H|
|CD80/B7-1||Human||CD80/B7-1 Protein, Recombinant||10698-H08H|
|CD80/B7-1||Human||CD80/B7-1 Protein, Recombinant||10698-HCCH|
|CD80/B7-1||Mouse||CD80/B7-1 Protein, Recombinant||50446-M08H|
|CD80/B7-1||Mouse||CD80 / B7-1 Protein, Recombinant||50446-M02H|
|Molecule||Application||Description //For Detailed Info. and Price------CLICK!||Cat. No|
|Human CD80/B7-1||WB, ELISA||CD80 / B7-1 Antibody||10698-RP01|
|Human CD80/B7-1||WB, ELISA||CD80 / B7-1 Antibody (Antigen Affinity Purified)||10698-RP02|
|Human CD80/B7-1||WB, ELISA||CD80 / B7-1 Antibody||10698-MM01|
|Human CD80/B7-1||FCM||CD80 / B7-1 Antibody ( FITC )||10698-MM01-F|
|Mouse CD80/B7-1||WB, ELISA||CD80 / B7-1 Antibody||50446-RP01|
|Mouse CD80/B7-1||WB, ELISA||CD80 / B7-1 Antibody (Antigen Affinity Purified)||50446-RP02|
CD80 / B7-1 Antibody Background
The B-lymphocyte activation antigen B7-1 (referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. Binding of this protein with CTLA-4 is involved in down-regulation of immune response.Recent study has revealed that contacts between B and T helper cells mediated by CD80 provide negative signal for the proliferation and IgG secretion of normal B cells and B cell lymphomas. In addition, B7-1 also plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages.CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.
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