CD80 / B7-1 Antibody

CD80 / B7-1 Antibody Datasheet

CD80 / B7-1 Antibody Product Information

• Immunogen: Recombinant Human CD80 / B7-1 Protein (Catalog #10698-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 01
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, human cell-derived, recombinant Human CD80 / B7-1 (rh CD80 / B7-1; Catalog #10698-H08H; Met 1- Asn 242; NP_005182.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD80 / B7-1 Antibody Usage Guide

• Specificity: Human CD80 / B7-1
• No cross-reactivity in ELISA with

  Human CD28

  Human B7-2

  Human B7-H1

  Mouse B7-H2

  Human PDL2

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human B7-1 in WB. Using a DAB detection system, the detection limit for Human B7-1 is approximately 20 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human B7-1. The detection limit for Human B7-1 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD80 / B7-1 Antibody Background

The B-lymphocyte activation antigen B7-1(referred to as B7), also known as CD80, is a member of cell surface immunoglobulin superfamily and is expressed on the surface of antigen-presenting cells including activated B cells, macrophages and dendritic cells. As costimulatory ligands, B7-1 which exists predominantly as dimer and the related protein B7-2, interact with the costimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells, and thus constitute one of the dominant pathways that regulate T cell activation and tolerance, cytokine production, and the generation of CTL. Binding of this protein with CTLA-4 is involved in down-regulation of immune response.Recent study has revealed that contacts between B and T helper cells mediated by CD80 provide negative signal for the proliferation and IgG secretion of normal B cells and B cell lymphomas. In addition, B7-1 also plays a role in induction of innate immune responses by activating NF-κB-signaling pathway in macrophages.CD80 is thus regarded as promising therapeutic targets for autoimmune diseases and various carcinomas.

References

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4. Bhatia, S. et al., 2005, Proc. Natl. Acad. Sci. 102: 15569-15574.
5. Sigal, L.J. et al., 1998, J. Immunol. 161: 2740-2745.
6. Khan, N. et al., 2007, Int. Immunol. 19:477-486.