Rat CD79B / B29 Protein (His Tag)

Cd79b molecule, immunoglobulin-associated beta Protein Datasheet

CD79B / B29 Protein Product Information

• Synonym: CD79B, B29/Ig-beta, Igb
• Protein Construction: A DNA sequence encoding the rat CD79B(O70153)(Met1-Asp158) was expressed, fused with a polyhistidine tag at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD79B / B29 Protein QC Testing

• Purity: > 90 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Val 26
• Molecular Mass: The recombinant rat CD79B comprises 144 amino acids and predicts a molecular mass of 16.8 kDa. The apparent molecular mass of the recombinant protein is approximately 28 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD79B / B29 protein

CD79B / B29 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD79B / B29 Protein Description

B-cell antigen receptor complex-associated protein beta chain, also known as B-cell-specific glycoprotein B29, Ig-beta, Immunoglobulin-associated B29 protein, CD79b and B29, is a single-pass type I membrane protein which contains one Ig-like V-type ( immunoglobulin-like ) domain and one ITAM domain. CD79b / B29 is required in cooperation with CD79A for initiation of the signal transduction cascade activated by the B-cell antigen receptor complex (BCR) which leads to internalization of the complex, trafficking to late endosomes and antigen presentation. CD79b / B29 enhances phosphorylation of CD79A, possibly by recruiting kinases which phosphorylate CD79A or by recruiting proteins which bind to CD79A and protect it from dephosphorylation. Defects in CD79b / B29 are the cause of agammaglobulinemia type 6 (AGM6) which is a primary immunodeficiency characterized by profoundly low or absent serum antibodies and low or absent circulating B cells due to an early block of B-cell development.

References

1. Mueller BS. et al., 1992, Eur J Immunol. 22: 1621-5.
2. Wood WJ. et al., 1993, Genomics. 16: 187-92.
3. Hashimoto S. et al., 1994, Immunogenetics. 40: 145-9.
4. Radaev S. et al., 2010, Structure. 18: 934-43.
5. Burkard TR. et al., 2011, BMC Syst Biol. 5: 17-17.