Anti-CD74 Antibody

Cat: 11091-RP02
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Anti-CD74 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD74 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human CD74
Immunogen
Recombinant Human CD74 protein (Catalog#11091-H07H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD74 extracellular domain (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln 73-Met 232). CD74 specific IgG was purified by human CD74 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD74 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  
IHC-P 1:500-1:2000  
IP 0.5-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD74 Antibody (Rabbit Polyclonal antibody) Images
Human CD74 Immunohistochemistry(IHC) 4470
Immunochemical staining of human CD74 in human rectal cancer with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human CD74 Immunohistochemistry(IHC) 4471
Immunochemical staining of human CD74 in human lung with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).
Human CD74 Immunohistochemistry(IHC) 4473
Immunochemical staining of human CD74 in human colon carcinoma with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Human CD74 Immunoprecipitation(IP) 8048

CD74 was immunoprecipitated using:

Lane A:0.5 mg HuT78 Whole Cell Lysate

2 µL anti-CD74 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD74 rabbit polyclonal antibody,at 1:100 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 34 kDa

Observed band size: 34 kDa

Human CD74 Western blot (WB) 13338

Anti-CD74 rabbit polyclonal antibody at 1:500 dilution

Lane A: Raji Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:34 kDa

Observed band size:34 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD74 Antibody Alternative Names
Anti-DHLAG Antibody;Anti-HLADG Antibody;Anti-Ia-GAMMA Antibody;Anti-II Antibody
CD74 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

Full Name
CD74 molecule, major histocompatibility complex, class II invariant chain
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
  • Moynes R, et al. (1997) A marker to distinguish atypical fibroxanthoma from malignant fibrous histiocytoma. Cancer. 79 (11): 2115-24.
  • Gabrielle FA, et al. (2008) Regulation of dendritic cell migration by CD74, the MHC class 2-associated invariant chain. Science. 322 (5908): 1705-10.
  • Leng L, et al. (2003) MIF signal transduction initiated by binding to CD74. JEM.197 (11): 1467-76.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD74 Antibody Rabbit Polyclonal WB 102339-T32
    Anti-CD74 Antibody (APC) Mouse Monoclonal FCM 11091-M101-A
    Anti-CD74 Antibody (PE) Mouse Monoclonal FCM 11091-MM01-P
    Anti-CD74 Antibody Rabbit Polyclonal ELISA 11091-RP01
    Anti-CD74 Antibody (PE) Mouse Monoclonal FCM 11091-M101-P
    Anti-CD74 Antibody Rabbit Polyclonal ELISA 11091-T16
    Anti-CD74 Antibody Mouse Monoclonal IHC-P,FCM 11091-MM01
    Anti-CD74 Antibody (APC) Mouse Monoclonal FCM 11091-MM01-A
    Anti-CD74 Antibody Rabbit Monoclonal ELISA,IHC-P 11091-R301
    Anti-CD74 Antibody Mouse Monoclonal FCM 11091-M101
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