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CD74 / DHLAG / p33 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CD74 Antibody Product Information
Immunogen:Recombinant Human CD74 protein (Catalog#11091-H07H)
Clone ID:
Ig Type:Rabbit IgG
Concentration:
Endotoxin:
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CD74 extracellular domain (rh CD74; Catalog#11091-H07H; NP_004346.1; Gln 73-Met 232). CD74 specific IgG was purified by human CD74 affinity chromatography.
Human CD74 Antibody Usage Guide
Specificity:Human CD74 / DHLAG / p33
Application:WB, ELISA, IHC-P, IP

WB: 1-10 μg/mL

ELISA: 0.5-1.0 μg/mL

This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Human CD74. The detection limit for Human CD74 is 0.00245 ng/well.

IHC-P: 0.1-2 μg/mL

IP: 0.5-2 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD74 Antibody WB Application Image
Human CD74 Antibody IHC Application Image
CD74 / DHLAG / p33 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human colon carcinoma with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
CD74 / DHLAG / p33 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human lung with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
CD74 / DHLAG / p33 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
[Click to enlarge image]
Caption:
Immunochemical staining of human CD74 in human rectal cancer with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
Human CD74 Antibody IP Application Image
Other CD74 Antibody Products
Reactivity: Human  
Application: ELISA  IHC-P  
  • Slide 1
  • Slide 1
11091-R301-50
11091-R301-100
50 µg 
100 µg 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
11091-MM01-A-50
11091-MM01-A-100
50 Tests 
100 Tests 
Add to Cart
Reactivity: Human  
Application: FCM  
  • Slide 1
11091-MM01-P-100
11091-MM01-P-50
100 Tests 
50 Tests 
Add to Cart
Reactivity: Human  
Application: ELISA  
    11091-T16-200
    11091-T16-100
    11091-T16-50
    200 µg 
    100 µg 
    50 µg 
    Add to Cart
    Reactivity: Human  
    Application: FCM  
    • Slide 1
    11091-M101-P-100
    11091-M101-P-50
    100 Tests 
    50 Tests 
    Add to Cart
    Reactivity: Human  
    Application: ELISA  
      11091-RP01-400
      11091-RP01-100
      11091-RP01-200
       
      100 µg 
      200 µg 
      Add to Cart
      Reactivity: Human  
      Application: FCM  
      • Slide 1
      11091-M101-A-100
      11091-M101-A-50
      100 Tests 
      50 Tests 
      Add to Cart
      Reactivity: Human  
      Application: IHC-P  
      • Slide 1
      • Slide 1
      • Slide 1
      11091-MM01-200
      11091-MM01-100
      11091-MM01-50
      200 µg 
      100 µg 
      50 µg 
      Add to Cart
      CD74 Background

      The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD74, also known as HLA class2 histocompatibility antigen gamma chain and HLA-DR antigens-associated invariant chain, is a polypeptide involved in the formation and transport of MHC class2 protein. CD74 is expressed by B cells, macrophages, and Reed-Sternberg cells. When MHC class 2 protein was in the rough ER, its peptide-binding cleft was blocked by CD74 to prevent it from interacting with the endogenous peptides. CD74 also serves to facilitate MHC class2's export from ER.

      Human CD74 References
    • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
    • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
    • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters.134 (2): 104-12.
    • Moynes R, et al. (1997) A marker to distinguish atypical fibroxanthoma from malignant fibrous histiocytoma. Cancer. 79 (11): 2115-24.
    • Gabrielle FA, et al. (2008) Regulation of dendritic cell migration by CD74, the MHC class 2-associated invariant chain. Science. 322 (5908): 1705-10.
    • Leng L, et al. (2003) MIF signal transduction initiated by binding to CD74. JEM.197 (11): 1467-76.
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      Catalog: 11091-RP02-50
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      All information of our products is subject to change without notice. Please refer to COA enclosed in shipped package for the newest information.
      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"