Anti-CD73 Antibody

Cat: 10904-MM05
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Anti-CD73 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD73 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD73
No cross-reactivity with Human cell lysate (293 cell line) in ELISA.
Recombinant Human CD73 / NT5E Protein (Catalog#10904-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD73 / NT5E (rh CD73 / NT5E; Catalog#10904-H08H; NP_002517.1; Met 1-Lys 547). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG2b Clone #8D10G7
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD73 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD73 Antibody (Mouse Monoclonal antibody) Images
Human CD73/NT5E Immunoprecipitation(IP) 6173

NT5E was immunoprecipitated using:

Lane A:0.5 mg HepG2 Whole Cell Lysate

Lane B:0.5 mg K562 Whole Cell Lysate

0.5 µL anti-NT5E mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-NT5E mouse monoclonal antibody,at 1:250 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 63 kDa

Observed band size: 63 kDa

Human CD73/NT5E Western blot (WB) 13302

Anti-NT5E mouse monoclonal antibody at 1:500 dilution

Lane A: HepG2 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:63 kDa

Observed band size:63 kDa

Anti-CD73 Antibody Alternative Names
Anti-CALJA Antibody;Anti-CD73 Antibody;Anti-E5NT Antibody;Anti-eN Antibody;Anti-eNT Antibody;Anti-NT Antibody;Anti-NT5 Antibody;Anti-NTE Antibody
CD73 Background Information

5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.

Full Name
5'-nucleotidase, ecto (CD73)
  • Resta R. et al., 1997, Cell Signal. 9 (2): 131-9.
  • Yamashita Y. et al., 1998, Eur J Immunol. 28 (10): 2981-90.
  • Louis NA. et al., 2008, J Immunol. 180 (6): 4246-55.
  • Grünewald JK. et al., 2010, J Inflamm. 7 (1): 10.
  • Product Description Host Clonality Application Catalog# (PDF)
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