**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
NT5E was immunoprecipitated using:
Lane A:0.5 mg HepG2 Whole Cell Lysate
Lane B:0.5 mg K562 Whole Cell Lysate0.5 µL anti-NT5E mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-NT5E mouse monoclonal antibody,at 1:250 dilutionSecondary antibody:
Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 63 kDa
Observed band size: 63 kDa
Anti-NT5E mouse monoclonal antibody at 1:500 dilution
Lane A: HepG2 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:63 kDa
Observed band size:63 kDa
5'-nucleotidase, also known as NT5E, NTE, and CD73, is a cell membrane protein which belongs to the 5'-nucleotidase family. CD73 is a glycosyl phosphatidylinositol (GPI) anchored purine salvage enzyme expressed on the surface of human T and B lymphocytes. CD73 catalyzes the conversion of purine and pyrimidine ribo- and deoxyribonucleoside monophosphates to the corresponding nucleosides. CD73 serves as a costimulatory molecule in activating T cells. CD73 generated adenosine functions in cell signalling in many physiologic systems, including intestinal epithelium, ischemic myocardium, and cholinergic synapses. CD73 might mediate lymphocyte-stromal cell interactions or condition the local microenvironment to facilitate lymphocyte development and/or function. In CD73-depleted cells, surface levels of the leukocyte adhesion molecules ICAM-1, VCAM-1 and E-selectin increase. CD73 produces extracellular adenosine, which then acts on G protein-coupled purigenic receptors to induce cellular responses. CD73 has also been reported to regulate expression of pro-inflammatory molecules in mouse endothelium.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD73 Antibody (FITC)||Mouse||Monoclonal||FCM||10904-MM07-F|
|Anti-CD73 Antibody (PE)||Mouse||Monoclonal||FCM||10904-MM07-P|