Rat CD7 Protein

Cd7 molecule Protein Datasheet

CD7 Protein Product Information

• Synonym: CD7
• Protein Construction: A DNA sequence encoding the rat CD7(B2RZ54)(Met1-Pro149) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD7 Protein QC Testing

• Purity: > 85% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 23
• Molecular Mass: The recombinant rat CD7/Fc is a disulfide-linked homodimer. The reduced monomer comprises 368 amino acids and has a predicted molecular mass of 41.1 kDa. The apparent molecular mass of the protein is approximately 48 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD7 protein

CD7 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD7 Protein Description

T-cell antigen CD7, also known as T-cell surface antigen Leu-9, and CD7, is a single-pass type I  membrane protein. It is expressed on human T and NK cells, as well as on cells in the early stages of T, B, and myeloid cell differentiation. CD7 is a T cell-associated antigen which appears early in ontogeny and persists on circulating T cells. It appears to have a significant role in T cell development and function. The precise mechanism by which this molecule mediates its effect is not known. CD7-mediated signals appear to augment function of adhesion molecules on NK cells, which may be involved in NK cell activation by providing both anchorage and costimulatory triggering. The CD7 molecule is a differentiation antigen found on the surface of T lymphocytes and also on a very minor fraction of acute nonlymphocytic leukemia (ANLL). CD7 is highly expressed on malignant immature T cells and is generally absent on malignant mature T cells, such as CD4+ Sezary leukemia and HTLV-1+ adult T-cell leukemia cells. Studies in CD7-deficient mice have provided insights into CD7 function, and demonstrated key roles for CD7 in regulating peripheral T and NK cell cytokine production and sensitivity to LPS-induced shock syndromes.

References

1. Rabinowich H. et al., 1994, J Immunol. 152 (2): 517-26.
2. Leta E. et al., 1995, Cell Immunol. 165 (1): 101-9.
3. Polskj JM. et al., 2000, Leuk Lymphoma. 39 (5-6): 633-9.
4. Stillwell R. et al., 2002, Immunol. Res. 24 (1): 31-52.
5. Murphy M. et al., 2002, Am J Dermatopathol. 24 (1): 6-16.