CD7 / LEU9 Antibody

Mouse CD7 / LEU9 Antibody

CD7 / LEU9 Antibody Product Information

• Immunogen: Recombinant mouse CD7 protein ( Catalog#50393-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 017
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse CD7 / LEU9 extracellular domain ( rM CD7 ; Catalog#50393-M08H ; NP_033984.1 ; Met 1 - Pro 150 ).

CD7 / LEU9 Antibody Usage Guide

• Specificity: Mouse CD7 / LEU9
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse CD7 in WB. Using a DAB detection system, the detection limit for mouse CD7 is approximately 2 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse CD7. The detection limit for mouse CD7 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD7 / LEU9 Antibody Background

T-cell antigen CD7, also known as T-cell surface antigen Leu-9, and CD7, is a single-pass type I membrane protein. It is expressed on human T and NK cells, as well as on cells in the early stages of T, B, and myeloid cell differentiation. CD7 is a T cell-associated antigen which appears early in ontogeny and persists on circulating T cells. It appears to have a significant role in T cell development and function. The precise mechanism by which this molecule mediates its effect is not known. CD7-mediated signals appear to augment function of adhesion molecules on NK cells, which may be involved in NK cell activation by providing both anchorage and costimulatory triggering. The CD7 molecule is a differentiation antigen found on the surface of T lymphocytes and also on a very minor fraction of acute nonlymphocytic leukemia (ANLL). CD7 is highly expressed on malignant immature T cells and is generally absent on malignant mature T cells, such as CD4+ Sezary leukemia and HTLV-1+ adult T-cell leukemia cells. Studies in CD7-deficient mice have provided insights into CD7 function, and demonstrated key roles for CD7 in regulating peripheral T and NK cell cytokine production and sensitivity to LPS-induced shock syndromes.

References

1. Rabinowich, H. et al., 1994, J Immunol. 152 (2): 517-26.
2. Leta, E. et al., 1995, Cell Immunol. 165 (1): 101-9.
3. Polskj, JM. et al., 2000, Leuk Lymphoma. 39 (5-6): 633-9.
4. Stillwell, R. et al., 2002, Immunol. Res. 24 (1): 31-52.
5. Murphy, M. et al., 2002, Am J Dermatopathol. 24 (1): 6-16.