Rat CD69 Gene cDNA Clone / ORF Clone

CD69 cDNA Clone Datasheet

CD69 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_134327.1
• cDNA Size: 600
• Gene Synonym: Cd69
• Source: Rat

CD69 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus Cd69 molecule DNA.
• Vector: pGEM-T Vector
• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD69 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD69 cDNA Clone Usage Protocol

1. Remove the marked edge in the center of the spot.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to a heating block at 95℃ for 15-30 min.
4. At the end of the incubation period remove the sample from the block and pulse vortex, or gently tap.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD69 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAATTCTG AAGAGTGTTC CATAACAGAA AATAGCTCTT CACATCTGGA GAGAGGGCAG
61       AGGGACCATG GCACCAGTGT ACATTTTGAG AAGCATCGTG AAGGATCCAT TCAAGTTCCT
121      ATCCCTTGTG CTGTGCTCGT AGTGGTCCTC ATCACTTCCT TAATTATCGC TCTCTTTGCC
181      TTAAGTGTGG GCAAGTACAA TTGTCCGGGC TTCTATGAGA ATCTGGAGTC ATTTGACCAC
241      CATGCTGCTT CCTGCAAGAA TGAGTGGTTT TCATACAACG GGAAATGTTA CTTTTTCTCC
301      ACCACAACCA AGACTTGGGC CTTGGCCCAA AAATCTTGTT CTGAAGATGA CGCTACCCTT
361      GCTGTTATTG ATTCGGAAAA GGACATGGCG TTTCTGAAGC GATATGCTGG TGGACTGAAA
421      CACTGGATTG GGCTGAGAAA CGAAGCTAGT CAGACATGGA AATGGGCAAA TGGCAAAGAA
481      TTTAACAGCT GGTTCAACGT GACAGGGTCT AAAAAGTGCG TGTCTCTGAA CCACACAGAT
541      GTTGCTTCTG TAGACTGCGA GGCAAACTTA CACTGGATCT GCAGCAAGGC CTCCCTATGA

 

Quality Control:cDNA sequencing results is available upon request.