Anti-CD69 Antibody

Cat: 11150-R074
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Anti-CD69 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD69 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD69
Recombinant Human CD69 Protein (Catalog#11150-H08H)
This antibody was obtained from a rabbit immunized with purified, recombinant Human CD69 (rh CD69; Catalog#11150-H08H; NP_001772.1; Ser 62-Lys 199).
Monoclonal Rabbit IgG Clone #074
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD69 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD69 Antibody (Rabbit Monoclonal antibody) Images
Human Peroxiredoxin 6/PRDX6 Immunoprecipitation(IP) 8075

CD69 was immunoprecipitated using:

Lane A:0.5 mg HL-60 Whole Cell Lysate

Lane B:0.5 mg Jurkat Whole Cell Lysate

0.5 µL anti-CD69 rabbit monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD69 rabbit monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 23 kDa

Observed band size: 23 kDa

Human CD69 / CLEC2C Western blot (WB) 13354

Anti-CD69 rabbit monoclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: HL60 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:23 kDa

Observed band size:23 kDa

Anti-CD69 Antibody Alternative Names
Anti-AIM Antibody;Anti-BL-AC/P26 Antibody;Anti-CLEC2C Antibody;Anti-EA1 Antibody;Anti-GP32/28 Antibody;Anti-MLR-3 Antibody
CD69 Background Information

Early activation antigen CD69, also known as activation inducer molecule (AIM), is a single-pass type II membrane protein. Recently, cDNA clones encoding human and mouse CD69 were isolated and showed CD69 to be a member of the C-type lectin superfamily. It is one of the earliest cell surface antigens expressed by T cells following activation. Once expressed, CD69 acts as a costimulatory molecule for T cell activation and proliferation. In addition to mature T cells, CD69 is inducibly expressed by immature thymocytes, B cells, natural killer (NK) cells, monocytes, neutrophils and eosinophils, and is constitutively expressed by mature thymocytes and platelets. CD69 is involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, and platelets. The structure, chromosomal localization, expression and function of CD69 suggest that it is likely a pleiotropic immune regulator , potentially important in the activation and differentiation of a wide variety of hematopoietic cells. This membrane molecule transiently expresses on activated lymphocytes, and its selective expression in inflammatory infiltrates suggests that it plays a role in the pathogenesis of inflammatory diseases. CD69 plays a crucial role in the pathogenesis of allergen-induced eosinophilic airway inflammation and hyperresponsiveness and that CD69 could be a possible therapeutic target for asthmatic patients.

Full Name
CD69 molecule
  • Ziegler SF, et al. (1994) The activation antigen CD69. Stem Cells. 12(5): 456-65.
  • Marzio R, et al. (1999) CD69 and regulation of the immune function. Immunopharmacol Immunotoxicol. 21(3): 565-82.
  • Lamana A, et al. (2006) The role of CD69 in acute neutrophil-mediated inflammation. Eur J Immunol. 36(10): 2632-8.
  • Miki-Hosokawa T, et al. (2009) CD69 controls the pathogenesis of allergic airway inflammation. J Immunol. 183(12): 8203-15.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD69 Antibody Rabbit Polyclonal ELISA 50731-T16
    Anti-CD69 Antibody Rabbit Polyclonal ELISA 11150-RP01
    Anti-CD69 Antibody Rabbit Polyclonal ELISA 11150-RP02
    Anti-CD69 Antibody Mouse Monoclonal ELISA,ELISA(Det) 11150-MM05
    Anti-CD69 Antibody Rabbit Monoclonal ELISA,ELISA(Cap) 11150-R006
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