Rat CD68 Gene cDNA Clone / ORF Clone

CD68 cDNA Clone Datasheet

CD68 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_001031638.1
• cDNA Size: 993
• Gene Synonym: MGC114377; Cd68
• Source: Rat

CD68 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus Cd68 molecule DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref. ID sequence except for the point mutation 84 C/T and 186 A/C not causing the amino acid variation.

CD68 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD68 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD68 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGGTTCC CTGTGTGTCT GACCTTGCTG GTACTGCTTG TAGCCCAAGG AACAGGGAAA
61       GACTGTCCTC ACAAAAAGGC TGCTACTCTT CTGCCATCCT TCACGGAGAC ACCTACAACC
121      ACCGGAAGCA CAGCAAGCCC TACGACCACC CACAGGCCCA CCACCACCAG CCACAGGCCC
181      ACCACAACCA GCCACAGGCC CACCACCACC AGCCACAGGC CCACCACCAC CAGCCACAGG
241      CCCACCACCA CCAGCCACAG GCCCACCACC ACCAGTCATG GGAATGCCAC AGTTTCTCCC
301      ACCACAAACA GTCCAGGCTT CTCCACTGTT GGCCCTCACC CTGGACCACC TCCGCCCTCA
361      CCAAGTCCTA GTCCAAGCTC TACTGGGGCT CTTGGAAACT ACACATGGAC TAATGGTTCC
421      CAGCCATGTG TTCAGCTCCA AGCCCAAATT CAAATTCGCA TCTTGTACCT GACCCAGGGT
481      GGAAAAAAGG CCTGGGGCCT CTCTGTATTG AACCCGAACA AAACCAAGGT CCAGGGGGGC
541      TGTGACAGTG CCCATCCCCA CTTGGCTCTC TCATTCCCTT ACGGACAGCT TACCTTTGGA
601      TTCAAACAGG ACCGACATCA GAGCCACAGT ACAGTCTACC TTAACTACAT GGCAGTGGAA
661      TACAATGTGT CCTTCCCACA AGCAGCACAG TGGACATTCT CAGCGCAGAA TTCATCTCTT
721      CAAGAGCTCC AAGCTCCCCT GGGCCAAAGC TTCTGTTGCG GAAATACAAG CATAGTTCTT
781      TCTCCAGCAA TTCACCTGGA CCTGCTCTCC CTGAGGCTGC AGGCTGCTCA GTTGCCGGAC
841      AAGGGACACT TCGGGCCATG CTTCTCTTGC GCCAGTGACC AATCTCTCTT GCTGCCTCTC
901      ATCATTGGCC TGGTCCTCCT GGGCCTCCTC ACCCTGGTGC TCATTGCCTT CTGCGTTACC
961      CGGAGACGAC AATCAACCTA CCAGCCCCTC TGA

 

Quality Control:cDNA sequencing results is available upon request.