CD64 / FCGR1A Protein (AVI Tag) , Biotinylated

CD64 / FCGR1A ( Biotinylated ) Protein

CD64 / FCGR1A ( Biotinylated ) Protein Product Information

• Synonym: FCGR1A, CD64, CD64A, FCRI, FLJ18345, IGFR1
• Protein Construction: A DNA sequence encoding the extracellular domain of human CD64 ( NP_000557.1 ) ( Met 1 - Thr 287 ) was fused with a C-terminal polyhistidine tagged AVI tag at the C-terminus The expressed protein was biotinylated in vivo by the Biotin-Protein ligase ( BirA enzyme ) which is co-expressed
• Source: Human
• Expression Host: Human Cells

CD64 / FCGR1A ( Biotinylated ) Protein QC Testing

• mMB / mMP: 1.09
• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 16
• Molecular Mass: The secreted recombinant human CD64 consists of 307 amino acids and has a predicted molecular mass of 34.4 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh CD64 is approximately 50 - 55 kDa due to glycosylation
• Formulation: Lyophilized from sterile PBS , pH 7.4
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: FCGR1A protein

CD64 / FCGR1A ( Biotinylated ) Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD64 / FCGR1A ( Biotinylated ) Protein Description

High affinity immunoglobulin gamma Fc receptor IA , also known as IgG Fc receptor IA (FCGR1A) and CD64, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity. CD64 belongs to the immunoglobulin superfamily. It is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. After binding IgG, CD64 interacts with an accessory chain known as the common gamma chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. There are three distinct genes in humans for CD64 called FcγRIA (CD64A), FcγRIB (CD64B), and FcγRIC (CD64C) that are located on chromosome 1. These three genes produce six different mRNA transcripts. Neutrophil CD64 is a highly sensitive marker for neonatal sepsis. Prospective studies incorporating CD64 into a sepsis scoring system are warranted.

References

1. Repp, R. et al., 1991, Blood. 78 (4): 885-9. 
2. Ernst, L. et al., 1992, J Biol Chem. 267 (22): 15692-700. 
3. Hulett, M. et al., 1998, Mol Immunol. 35 (14-15): 989-96. 
4. Nimmerjahn, F. et al., 2006, Immunity. 24 (1): 19-28. 
5. Bhandari, V. et al., 2008, Pediatrics. 121 (1): 129-34.
6. Thepen, T. et al., 2009, Curr Pharm Des. 15 (23): 2712-8.