Anti-CD64 Antibody

Cat: 50086-R001
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All CD64 Reagents
Anti-CD64 Antibody (Rabbit Monoclonal antibody) General Information
Product name
Anti-CD64 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse
Specificity
Mouse CD64
Has cross-reactivity in ELISA with Human CD64 / FCGR1A
No cross-reactivity in ELISA with Mouse CD16-2 /FCGR4 Mouse FCGR2B / CD32 Mouse FCGR3 / CD16 Human CD16a / FCGR3A Human CD16b / FCGR3B Human CD32a / FCGR2A Human CD32b / FCGR2B
Immunogen
Recombinant Mouse FCGR1 protein (Catalog#50086-M08H)
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR1 extracellular domain (rM FCGR1; Catalog#50086-M08H; NP_034316.1; Met 1-Pro 297).
Source
Monoclonal Rabbit IgG Clone #1
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD64 Antibody (Rabbit Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:500-1:2500  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD64 Antibody (Rabbit Monoclonal antibody) Images
Mouse CD64/Fc gamma RI Immunohistochemistry(IHC) 3294
Immunochemical staining of mouse FCGR1 in mouse spleen with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).
Mouse CD64/Fc gamma RI Immunohistochemistry(IHC) 3296
Immunochemical staining of mouse FCGR1 in mouse liver with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to Kupffer cell.
Mouse CD64/Fc gamma RI Immunohistochemistry(IHC) 3298
Immunochemical staining of mouse FCGR1 in mouse brain with rabbit monoclonal antibody (1:1000, formalin-fixed paraffin embedded sections). Positive staining was localized to microglia.
Mouse CD64/Fc gamma RI Western blot (WB) 18975

Anti-FCGR1 rabbit monoclonal antibody at 1:500 dilution

Lane A: RAW264.7 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:45 kDa

Observed band size:40 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD64 Antibody Alternative Names
Anti-AI323638 Antibody;Anti-AV092959 Antibody;Anti-CD64 Antibody;Anti-FcgammaRI Antibody;Anti-IGGHAFC Antibody
CD64 Background Information

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Full Name
Fc receptor, IgG, high affinity I
References
  • Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
  • Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
  • Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD64 Antibody Rabbit Monoclonal ELISA 80016-R015
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 80016-R005-P
    Anti-CD64 Antibody Mouse Monoclonal ELISA 80016-MM01
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-T16
    Anti-CD64 Antibody Rabbit Monoclonal FCM 80016-R005
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 80016-R005-A
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-RP02
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-RP01
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 80016-R005-F
    Anti-CD64 Antibody Rabbit Polyclonal WB,ELISA 50086-RP02
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 50086-R027-P
    Anti-CD64 Antibody (PerCP) Rabbit Monoclonal FCM 50086-R027-C
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 50086-RP01
    Anti-CD64 Antibody Rabbit Monoclonal ELISA,IHC-P 50086-R008
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 50086-R027-F
    Anti-CD64 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 50086-R027
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 50086-R027-A
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 10256-R407-F
    Anti-CD64 Antibody Rabbit Monoclonal ELISA,FCM 10256-R407
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 10256-R401-F
    Anti-CD64 Antibody Rabbit Monoclonal FCM 10256-R401
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 10256-R401-A
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 10256-R401-P
    Anti-CD64 Antibody Rabbit Polyclonal WB,ELISA 10256-RP02
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 10256-RP01
    Anti-CD64 Antibody Mouse Monoclonal ELISA 10256-MM06
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 10256-T16
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