FCGR1 / CD64 Antibody

Mouse High Affinity Immunoglobulin Gamma Fc Receptor I Antibody

FCGR1 / CD64 Antibody Product Information

• Immunogen: Recombinant mouse FCGR1 protein ( Catalog#50086-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 1
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse FCGR1 extracellular domain ( rM FCGR1 ; Catalog#50086-M08H ; NP_034316.1 ; Met 1 - Pro 297 ).

FCGR1 / CD64 Antibody Usage Guide

• Specificity: Mouse FCGR1 / CD64
• Has cross-reactivity in ELISA with

  Human CD64 / FCGR1A

  No cross-reactivity in ELISA with

  Mouse CD16-2 / FCGR4

  Mouse FCGR2B / CD32

  Mouse FCGR3 / CD16

  Human CD16a / FCGR3A

  Human CD16b / FCGR3B

  Human CD32a / FCGR2A

  Human CD32b / FCGR2B

• Immunochemistry: IHC-P: 2-10 μg/mL

  

  Immunochemical staining of mouse FCGR1 in mouse spleen with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).

  

  Immunochemical staining of mouse FCGR1 in mouse brain with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to microglia.

  Immunochemical staining of mouse FCGR1 in mouse liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to Kupffer cell.

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse FCGR1 in WB. Using a DAB detection system, the detection limit for mouse FCGR1 is approximately 0.125 ng/lane under non-reducing conditions and reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse FCGR1. The detection limit for mouse FCGR1 is approximately 0.00245 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

FCGR1 / CD64 Antibody Background

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

References

1. Perussia, B. et al., 1983, J Exp Med. 158 (4): 1092-113.
2. Repp, R. et al., 1991, Blood. 78 (4): 885-9.
3. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-83.
4. Takai, T., 2002, Nature Rev. Immunol. 2: 580-92.
5. Barnes, N. et al., 2002, Immunity 16: 379-89.