|Recombinant Mouse FCGR1 protein (Catalog#50086-M08H)|
|0.2 μm filtered solution in PBS with 5% trehalose|
|This antibody was obtained from a rabbit immunized with purified, recombinant Mouse FCGR1 extracellular domain (rM FCGR1; Catalog#50086-M08H; NP_034316.1; Met 1-Pro 297).|
|Mouse FCGR1 / CD64|
Has cross-reactivity in ELISA with
Human CD64 / FCGR1A
No cross-reactivity in ELISA with
Mouse CD16-2 /FCGR4
Mouse FCGR2B / CD32
Mouse FCGR3 / CD16
Human CD16a / FCGR3A
Human CD16b / FCGR3B
Human CD32a / FCGR2A
Human CD32b / FCGR2B
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FCGR1. The detection limit for Mouse FCGR1 is approximately 0.00245 ng/well.
IHC-P: 2-10 μg/mL
|This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.|
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.