Anti-CD64 Antibody

Cat: 10256-RP02
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All CD64 Reagents
Anti-CD64 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD64 Antibody
Validated applications
WB,ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD64
Immunogen
Recombinant Human CD64 protein (Catalog#10256-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD64 (rh CD64; Catalog#10256-H08H; NP_000557.1; Met 1-Pro 288). CD64 specific IgG was purified by human CD64 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD64 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD64 Antibody (Rabbit Polyclonal antibody) Images
Human CD64/Fc gamma RI Western blot (WB) 13153

Anti-CD64 rabbit polyclonal antibody at 1:500 dilution

Lane A: THP1 Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:43 kDa

Observed band size:42 kDa

(We are unsure as to the identity of these extra bands.)

Anti-CD64 Antibody Alternative Names
Anti-CD64 Antibody;Anti-Fc gamma RI Antibody
CD64 Background Information

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

Full Name
Fc receptor, IgG, high affinity I
References
  • Allen J.M., et al.,(1988), Nucleotide sequence of three cDNAs for the human high affinity Fc receptor (FcRI). Nucleic Acids Res. 16:11824-11824.
  • Allen J.M., et al., (1989), Isolation and expression of functional high-affinity Fc receptor complementary DNAs.Science 243:378-381.
  • Porges A.J., et al.,(1992), Novel Fc gamma receptor I family gene products in human mononuclear cells.J. Clin. Invest. 90:2102-2109.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-RP02
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 80016-R005-A
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-T16
    Anti-CD64 Antibody Mouse Monoclonal ELISA 80016-MM01
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 80016-R005-P
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 80016-RP01
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 80016-R005-F
    Anti-CD64 Antibody Rabbit Monoclonal ELISA 80016-R015
    Anti-CD64 Antibody Rabbit Monoclonal FCM 80016-R005
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 50086-R027-A
    Anti-CD64 Antibody Rabbit Polyclonal WB,ELISA 50086-RP02
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 50086-RP01
    Anti-CD64 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 50086-R027
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 50086-R027-P
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 50086-R027-F
    Anti-CD64 Antibody Rabbit Monoclonal ELISA,IHC-P 50086-R008
    Anti-CD64 Antibody (PerCP) Rabbit Monoclonal FCM 50086-R027-C
    Anti-CD64 Antibody Rabbit Monoclonal WB,ELISA,IHC-P 50086-R001
    Anti-CD64 Antibody Rabbit Monoclonal FCM 10256-R401
    Anti-CD64 Antibody (APC) Rabbit Monoclonal FCM 10256-R401-A
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 10256-RP01
    Anti-CD64 Antibody Rabbit Monoclonal ELISA,FCM 10256-R407
    Anti-CD64 Antibody (PE) Rabbit Monoclonal FCM 10256-R401-P
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 10256-R401-F
    Anti-CD64 Antibody Mouse Monoclonal ELISA 10256-MM06
    Anti-CD64 Antibody Rabbit Polyclonal ELISA 10256-T16
    Anti-CD64 Antibody (FITC) Rabbit Monoclonal FCM 10256-R407-F
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