CD64 / FCGR1 Antibody

High Affinity Immunoglobulin Gamma Fc Receptor IA Antibody

CD64 / FCGR1A Antibody Product Information

• Immunogen: Recombinant human CD64 protein ( Catalog#10256-H08H )
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, human cell-derived, recombinant human CD64 ( rh CD64 ; Catalog#10256-H08H ; NP_000557.1 ; Met 1 - Pro 288 ). Total IgG was purified by Protein A affinity chromatography.

CD64 / FCGR1A Antibody Usage Guide

• Specificity: Human CD64 / FCGR1A
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD64 in WB
• Direct ELISA: This antibody can be used at 0.5 -1.0 μg/mL with the appropriate secondary reagents to detect human CD64. The detection limit for human CD64 is 0.0049 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD64 / FCGR1A Antibody Background

High affinity immunoglobulin gamma Fc receptor IA , also known as IgG Fc receptor IA (FCGR1A) and CD64, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity. CD64 belongs to the immunoglobulin superfamily. It is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. After binding IgG, CD64 interacts with an accessory chain known as the common gamma chain (γ chain), which possesses an ITAM motif that is necessary for triggering cellular activation. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. There are three distinct genes in humans for CD64 called FcγRIA (CD64A), FcγRIB (CD64B), and FcγRIC (CD64C) that are located on chromosome 1. These three genes produce six different mRNA transcripts. Neutrophil CD64 is a highly sensitive marker for neonatal sepsis. Prospective studies incorporating CD64 into a sepsis scoring system are warranted

References

1. Repp, R. et al., 1991, Blood. 78 (4): 885-9. 
2. Ernst, L. et al., 1992, J Biol Chem. 267 (22): 15692-700. 
3. Hulett, M. et al., 1998, Mol Immunol. 35 (14-15): 989-96. 
4. Nimmerjahn, F. et al., 2006, Immunity. 24 (1): 19-28. 
5. Bhandari, V. et al., 2008, Pediatrics. 121 (1): 129-34.
6. Thepen, T. et al., 2009, Curr Pharm Des. 15 (23): 2712-8.