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CD64 / FCGR1 Antibody (FITC), Rabbit MAb

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CD64/FCGR1Antibody Product Information
Antigen:Recombinant Mouse CD64 / FCGR1 protein (Catalog#50086-M08H)
Clone ID:027
Ig Type:Rabbit IgG
Concentration:10 μl/Test, 0.1 mg/ml
Formulation:Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Preparation:This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD64 / FCGR1 (rM CD64 / FCGR1; Catalog#50086-M08H; NP_034316.1; Met 1-Pro 297) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.
CD64/FCGR1Antibody Usage Guide
Specificity:Mouse CD64 / FCGR1
Has cross-reactivity in ELISA with
Human CD16b
Human CD64
No cross-reactivity in ELISA with
Mouse FCGR4
Mouse FCGR2B
Human CD16a
Human CD32a
Human CD32b
Mouse FCGR3
Application:FCM
Storage:This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Images

Profile of anti-FCGR1 (CD64) reactivity on Raw264.7 cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with 5 μg of mouse IgG/106 cells for 1 hour at 4 ℃ prior to staining, washed, then stained with FITC Rabbit anti-FCGR1 (CD64).

Background

Mouse high affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membrane glycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of a signal peptide that allows its transport to the surface of a cell, three extracellular immunoglobulin domains of the C2-type that it uses to bind antibody, a hydrophobic transmembrane domain, and a short cytoplasmic tail. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

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