CD64 / FCGR1 Antibody ( FITC )

CD64 / FCGR1 Antibody Datasheet

CD64 / FCGR1 Antibody Product Information

• Immunogen: Recombinant Mouse CD64 / FCGR1 protein (Catalog#50086-M08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 027
• Ig Type: Rabbit IgG
• Concentration: 10 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.09% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD64 / FCGR1 (rM CD64 / FCGR1; Catalog#50086-M08H; NP_034316.1; Met 1-Pro 297) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD64 / FCGR1 Antibody Usage Guide

• Specificity: Mouse CD64 / FCGR1
• Has cross-reactivity in ELISA with
  

  Human CD16b

  Human CD64

  No cross-reactivity in ELISA with
  

  Mouse FCGR4

  Mouse FCGR2B

  Human CD16a

  Human CD32a

  Human CD32b

  Mouse FCGR3

• Flow Cytometry:
  

  Profile of anti-FCGR1 (CD64) reactivity on Raw264.7 cells analyzed by flow cytometry. Cells should be Fc-blocked by treatment with 5 μg of mouse IgG/106 cells for 1 hour at 4 ℃ prior to staining, washed, then stained with FITC Rabbit anti-FCGR1 (CD64).

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD64 / FCGR1 Antibody Background

High affinity immunoglobulin gamma Fc receptor I, also known as FCGR1 and CD64, is an integral membraneglycoprotein and a member of the immunoglobulin superfamily. CD64 is a high affinity receptor for the Fc region of IgG gamma and functions in both innate and adaptive immune responses. Receptors that recognize the Fc portion of IgG function in the regulation of immune response and are divided into three classes designated CD64, CD32, and CD16. CD64 is structurally composed of asignal peptidethat allows its transport to the surface of a cell, threeextracellularimmunoglobulin domainsof the C2-type that it uses to bind antibody, a hydrophobictransmembrane domain, and a short cytoplasmic tail. CD64 isconstitutivelyfound on only macrophages and monocytes, but treatment of polymorphonuclear leukocyteswith cytokines likeIFNγandG-CSFcan induce CD64 expression on these cells. The inactivation of the mouse CD64 resulted in a wide range of defects in antibody Fc-dependent functions. Mouse CD64 is an early participant in Fc-dependent cell activation and in the development of immune responses.

References

1. Perussia, B. et al., 1983,J Exp Med. 158 (4): 1092-113.
2. Repp, R. et al., 1991, Blood. 78 (4): 885-9.
3. Ravetch, J. et al., 2001, Annu. Rev. Immunol. 19: 275-83.
4. Takai, T., 2002, Nature Rev. Immunol. 2: 580-92.
5. Barnes, N. et al., 2002, Immunity 16: 379-89.