Sus scrofa (Pig) CD63 / Tspan-30 / Tetraspanin-30 Protein

Cd63 molecule Protein Datasheet

CD63 / Tspan-30 / Tetraspanin-30 Protein Product Information

• Synonym: CD63
• Protein Construction: A DNA sequence encoding the Sus scrofa (Pig) CD63 (XP_003355519.1) (Arg 108-Phe 204) was fused with the human IgG1 Fc region at the N-terminus.
• Source: Sus scrofa (Pig)
• Expression Host: Human Cells

CD63 / Tspan-30 / Tetraspanin-30 Protein QC Testing

• Purity: > 95 % as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gln 22
• Molecular Mass: The secreted recombinant Sus scrofa (Pig) CD63/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 338 amino acids and has a predicted molecular mass of 38.2 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions due to glycosylation.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD63 / Tspan-30 / Tetraspanin-30 protein

CD63 / Tspan-30 / Tetraspanin-30 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD63 / Tspan-30 / Tetraspanin-30 Protein Description

CD63 antigen, also known as Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, Ocular melanoma-associated antigen, Tetraspanin-30, CD63 and Tspan-30, is a multi-pass membrane protein which belongs to the tetraspanin (TM4SF) family. Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins characterized by four transmembrane domains. Tetraspanins participate in a variety of cellular processes, like cell activation, adhesion, differentiation and tumour invasion. At the cell surface, tetraspanins form networks with a wide diversity of proteins called tetraspanin-enriched microdomains (TEMs). CD63 / Tspan-30 was the first characterized tetraspanin. In addition to its presence in TEMs, CD63 / Tspan-30 is abundantly present in late endosomes and lysosomes. CD63 / Tspan-30 is expressed in melanoma and appears to be involved in regulation of motility and adhesion of melanoma cells. CD63 / Tspan-30 is associated with early stages of melanoma tumor progression. It may play a role in growth regulation. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). which is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects.

References

1. Smith M. et al., 1997, Melanoma Res. 7 (2): S163-70.
2. Chen Z. et al., 2004, Int J Mol Med. 14 (4): 517-27.
3. Iida J. et al., 2005, J Transl Med. 3: 42.
4. K?llquist L. et al., 2008, Blood. 112 (8): 3444-54.
5. Pols MS. et al., 2009, Exp Cell Res. 315 (9): 1584-92.