CD63 Antibody

CD63 Antibody Datasheet

CD63 Antibody Product Information

• Immunogen: Recombinant Human CD63 protein (Catalog#11271-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 10
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD63 (rh CD63; Catalog#11271-H08H; P08962-1; Ala 103-Val 203). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD63 Antibody Usage Guide

• Specificity: Human CD63
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Direct ELISA: This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human CD63. The detection limit for Human CD63 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD63 Antibody Background

CD63 antigen, also known as Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, Ocular melanoma-associated antigen, Tetraspanin-30, CD63 and Tspan-30, is a multi-pass membrane protein which belongs to thetetraspanin (TM4SF) family. Tetraspanins comprise a large superfamily of cell surface-associated membrane proteins characterized by four transmembrane domains. Tetraspanins participate in a variety of cellular processes, like cell activation, adhesion, differentiation and tumour invasion. At the cell surface, tetraspanins form networks with a wide diversity of proteins called tetraspanin-enriched microdomains (TEMs). CD63 / Tspan-30 was the first characterized tetraspanin. In addition to its presence in TEMs, CD63 / Tspan-30 is abundantly present in late endosomes and lysosomes. CD63 / Tspan-30 is expressed in melanoma and appears to be involved in regulation of motility and adhesion of melanoma cells. CD63 / Tspan-30 is associated with early stages of melanoma tumor progression. It may play a role in growth regulation. Lack of expression of CD63 in platelets has been observed in a patient with Hermansky-Pudlak syndrome (HPS). which is a genetically heterogeneous, rare, autosomal recessive disorder characterized by oculocutaneous albinism, bleeding due to platelet storage pool deficiency, and lysosomal storage defects.

References

1. Smith, M. et al., 1997, Melanoma Res. 7 Suppl 2: S163-70.
2. Chen, Z. et al., 2004, Int J Mol Med. 14 (4): 517-27.
3. Iida,J. et al., 2005, J Transl Med. 3: 42.
4. Kallquist,L. et al., 2008,Blood. 112 (8): 3444-54.
5. Pols, M.S. et al., 2009, Exp Cell Res. 315 (9): 1584-92.