CD6 Antibody

CD6 Antibody Datasheet

CD6 Antibody Product Information

• Immunogen: Recombinant Mouse CD6 protein (Catalog#50711-M08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 309
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Mouse CD6 (rM CD6; Catalog#50711-M08H; Q91WN5; Met1-Gly396).

CD6 Antibody Usage Guide

• Specificity: Mouse CD6
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse CD6. The detection limit for Mouse CD6 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD6 Antibody Background

T-cell differentiation antigen CD6, also known as TP120 and CD6, is a single-pass type I membrane protein which contains threeSRCR domains. CD6 / TP120 is a cell surface glycoprotein expressed primarily on T cells, it may function as a costimulatory molecule and may play a role in autoreactive immune responses. CD6 / TP120 is expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. CD6 ligand termed CD166 (previously known as activated leukocyte cell adhesion molecule, ALCAM) has been identified and shown to be expressed on activated T cells, B cells, thymic epithelium, keratinocytes, and in rheumatoid arthritis synovial tissue. CD6 / TP120 binds to activated leukocyte cell adhesion molecule (CD166), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development. CD6 / TP120 partially associates with the TCR / CD3 complex and colocalizes with it at the center of the mature immunological synapse (IS) on T lymphocytes. During thymic development CD6-dependent signals may contribute both to thymocyte survival, and to the overall functional avidity of selection in both man and mouse.

References

1. Joo,Y.S. et al., 2000,Arthritis Rheum. 43 (2): 329-35.
2. Singer,N.G. et al., 2002, Int Immunol. 14 (6): 585-97.
3. Gimferrer,I. et al.,2005,J Immunol. 175 (3): 1406-14.
4. Alonso,R. et al.,2010, J Autoimmun. 35 (4): 336-41.