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CD5L ELISA Pair Set, Mouse ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK50020 |
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Mouse CD5L ELISA Pair Set
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Order or Inquire for CD5L ELISA Pair Set |
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High affinity Mouse CD5L ELISA Pair Set |
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Detection limit - 6.25 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Mouse CD5L ELISA Pair Set Related Products & Topics
Related Areas:
Immunology>>Innate Immunity>>Monocyte/Macrophage>>Scavenger Receptor>>CD5L
Immunology>>Cluster of Differentiation>>B Cell CD Antigen>>CD5L
Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Other>>CD5L
Cardiovascular>>Lipid Metabolism>>Scavenger Receptor>>CD5L
Proteins:
| Molecule | Species | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| CD5L | Human | CD5L Protein, Recombinant | 10791-H08H |
| CD5L | Mouse | CD5L Protein, Recombinant | 50020-M08H |
Antibodies:
| Molecule | Application | Description //For Detailed Info. and Price------CLICK! | Cat. No |
| Mouse CD5L |
WB | Rabbit Monoclonal Antibody | 50020-R010 |
| Mouse CD5L |
ELISA | Rabbit Monoclonal Antibody | 50020-R038 |
| Mouse CD5L |
WB, ELISA | Rabbit Polyclonal Antibody | 50020-RP01 |
| Mouse CD5L |
WB, ELISA | Rabbit Polyclonal Antibody (Antigen Affinity Purified) | 50020-RP02 |
Background
CD5L, also known as CD5 antigen-like, is a soluble protein belonging to group B of the scavenger receptor cysteine-rich (SRCR) superfamily and contains three SRCR domains. It is a secreted glycoprotein and expressed by macrophages presentin lymphoid tissues (spleen, lymph node, thymus, and bone marrow). It binds to myelomonocytic and lymphoid cells and may play an important role in the regulation of the innate and adaptive immune systems. CD5L functions as a pattern recognition molecule by binding both lipoteichoic acid (LTA) on Gram positive and lipopolysaccharide (LPS) on Gram negative bacteria. and the SRCR domain 1 of CD5L retains both the LPS and LTA binding activities. In addtion, it is revealed that CD5L seems to play a role as an inhibitor of apoptosis.
