Rat CD59 Gene cDNA Clone / ORF Clone

CD59 cDNA Clone Datasheet

CD59 cDNA Clone Product Information

• Gene Bank Ref.ID: NM_012925.1
• cDNA Size: 381
• Gene Synonym: Cd59a; Cd59b; MACIF; MACIP; MAC-IP; Cd59
• Source: Rat

CD59 cDNA Clone Description

• cDNA Description: ORF Clone of Rattus norvegicus CD59 molecule, complement regulatory protein DNA.
• Vector: Cloning Vector: pGEM-T Vector

  Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG

• Sequence Description: Identical with the Gene Bank Ref.ID sequence.

CD59 cDNA Clone Shipping

1. 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
2. It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD59 cDNA Clone Usage Protocol

1. Cut along the circle edge of the spot (arrow points to) to get the filter paper.
2. Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
3. Transfer the tube to incubation at 95℃ for 15-30 min.
4. At the end of the incubation pulse vortex, or gently tap the tube.
5. Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD59 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGAGAGCTC GGAGGGGATT CATCTTACTC CTGCTTCTGG CTGTCCTCTG TTCCACAGGT
61       GTTAGCCTCA GATGCTACAA CTGTTTAGAC CCGGTTTCTT CATGCAAAAC AAACAGCACT
121      TGCTCTCCTA ACCTGGATGC TTGTCTTGTT GCTGTATCCG GAAAGCAAGT CTATCAACAG
181      TGTTGGAGAT TTTCGGATTG TAATGCCAAG TTCATTTTGA GCCGACTAGA AATCGCAAAC
241      GTACAATACA GATGCTGCCA GGCGGACTTG TGTAACAAAA GCTTCGAAGA CAAGCCAAAC
301      AATGGGGCAA TCTCCCTATT GGGGAAGACA GCGTTGCTGG TGACCTCGGT TCTGGCGGCC
361      ATTTTGAAGC CTTGTTTCTA A

 

Quality Control:cDNA sequencing results is available upon request.