Rat CD59 / CD59A / MAC-IP Protein

CD59 molecule, complement regulatory Protein Datasheet

CD59 / CD59A / MAC-IP Protein Product Information

• Synonym: CD59
• Protein Construction: A DNA sequence encoding the rat CD59 (P27274) (Met1-Asn100) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD59 / CD59A / MAC-IP Protein QC Testing

• Purity: (55.3+31.4)% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Leu 23
• Molecular Mass: The recombinant rat CD59 /Fc is a disulfide-linked homodimer. The reduced monomer comprises 319 amino acids and has a predicted molecular mass of 35.8 kDa. The apparent molecular mass of the protein is approximately 40 and 43 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD59 / CD59A / MAC-IP protein

CD59 / CD59A / MAC-IP Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD59 / CD59A / MAC-IP Protein Description

CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

References

1. Fletcher CM. et al., 1994, Structure. 2: 185-99.
2. Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
3. Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
4. Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
5. Picariello G. et al., 2008, Proteomics 8: 3833-47.
6. Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.