Anti-CD59 Antibody

Cat: 12474-RP02
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Anti-CD59 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD59 Antibody
Validated applications
WB,ELISA,IP
Species reactivity
Reacts with: Human
Specificity
Human CD59
Immunogen
Recombinant Human CD59 protein (Catalog#12474-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD59 (rh CD59; Catalog#12474-H08H; P13987-1; Met 1-Glu 101). CD59 specific IgG was purified by Human CD59 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD59 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IP 0.1-0.5 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD59 Antibody (Rabbit Polyclonal antibody) Images
Human CD59 Immunoprecipitation(IP) 8141

CD59 was immunoprecipitated using:

Lane A:0.5 mg PC-3 Whole Cell Lysate

0.5 µL anti-CD59 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD59 rabbit polyclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 18 kDa

Observed band size: 18 kDa

Human CD59 Western blot (WB) 13496

Anti-CD59 rabbit polyclonal antibody at 1:500 dilution

Lane A: PC3 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:14 kDa

Observed band size:18 kDa

Anti-CD59 Antibody Alternative Names
Anti-16.3A5 Antibody;Anti-1F5 Antibody;Anti-EJ16 Antibody;Anti-EJ30 Antibody;Anti-EL32 Antibody;Anti-FLJ38134 Antibody;Anti-FLJ92039 Antibody;Anti-G344 Antibody;Anti-HRF-20 Antibody;Anti-HRF20 Antibody;Anti-MAC-IP Antibody;Anti-MACIF Antibody;Anti-MEM43 Antibody;Anti-MGC2354 Antibody;Anti-MIC11 Antibody;Anti-MIN1 Antibody;Anti-MIN2 Antibody;Anti-MIN3 Antibody;Anti-MIRL Antibody;Anti-MSK21 Antibody;Anti-p18-20 Antibody
CD59 Background Information

CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

Full Name
CD59 molecule
References
  • Fletcher CM. et al., 1994, Structure. 2: 185-99.
  • Rudd PM. et al., 1997, J Biol Chem. 272: 7229-44.
  • Kimberley FC. et al., 2007, Mol Immunol. 44 (1-3): 73-81.
  • Gong Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
  • Picariello G. et al., 2008, Proteomics 8: 3833-47.
  • Heibeck TH. et al., 2009, J Proteome Res. 8: 3852-61.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD59 Antibody Rabbit Polyclonal ELISA 90188-RP01
    Anti-CD59 Antibody Rabbit Polyclonal ELISA 90188-T16
    Anti-CD59 Antibody Rabbit Monoclonal FCM 80299-R014
    Anti-CD59 Antibody (FITC) Rabbit Monoclonal FCM 80299-R001-F
    Anti-CD59 Antibody Rabbit Polyclonal ELISA 80299-RP01
    Anti-CD59 Antibody Rabbit Polyclonal WB,ELISA,IP 80299-T52
    Anti-CD59 Antibody Rabbit Monoclonal FCM 80299-R001
    Anti-CD59 Antibody (FITC) Rabbit Monoclonal FCM 80299-R014-F
    Anti-CD59 Antibody Rabbit Monoclonal ELISA(Cap) 80299-R002
    Anti-CD59 Antibody Rabbit Monoclonal ELISA 80299-R003
    Anti-CD59 Antibody (PE) Rabbit Monoclonal FCM 80299-R001-P
    Anti-CD59 Antibody (PE) Rabbit Monoclonal FCM 80299-R014-P
    Anti-CD59 Antibody Rabbit Polyclonal ELISA 80299-T16
    Anti-CD59 Antibody (APC) Rabbit Monoclonal FCM 80299-R001-A
    Anti-CD59 Antibody (APC) Rabbit Monoclonal FCM 80299-R014-A
    Anti-CD59 Antibody (PerCP) Rabbit Monoclonal FCM 12474-R029-C
    Anti-CD59 Antibody Rabbit Monoclonal ELISA(Cap) 12474-R015
    Anti-CD59 Antibody (FITC) Rabbit Monoclonal FCM 12474-R029-F
    Anti-CD59 Antibody (APC) Rabbit Monoclonal FCM 12474-R029-A
    Anti-CD59 Antibody Rabbit Polyclonal ELISA 12474-RP01
    Anti-CD59 Antibody Rabbit Monoclonal ELISA(Det) 12474-R031
    Anti-CD59 Antibody (PE) Rabbit Monoclonal FCM 12474-R029-P
    Anti-CD59 Antibody Rabbit Monoclonal ELISA,FCM 12474-R029
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