|IP||0.1-0.5 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
CD59 was immunoprecipitated using:
Lane A:0.5 mg PC-3 Whole Cell Lysate0.5 µL anti-CD59 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CD59 rabbit polyclonal antibody,at 1:500 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 18 kDa
Observed band size: 18 kDa
Anti-CD59 rabbit polyclonal antibody at 1:500 dilution
Lane A: PC3 Whole Cell LysateLysates/proteins at 30 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:14 kDa
Observed band size:18 kDa
CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains one UPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD59 Antibody (FITC)||Rabbit||Monoclonal||FCM||80299-R001-F|
|Anti-CD59 Antibody (FITC)||Rabbit||Monoclonal||FCM||80299-R014-F|
|Anti-CD59 Antibody (PE)||Rabbit||Monoclonal||FCM||80299-R001-P|
|Anti-CD59 Antibody (PE)||Rabbit||Monoclonal||FCM||80299-R014-P|
|Anti-CD59 Antibody (APC)||Rabbit||Monoclonal||FCM||80299-R001-A|
|Anti-CD59 Antibody (APC)||Rabbit||Monoclonal||FCM||80299-R014-A|
|Anti-CD59 Antibody (PerCP)||Rabbit||Monoclonal||FCM||12474-R029-C|
|Anti-CD59 Antibody (FITC)||Rabbit||Monoclonal||FCM||12474-R029-F|
|Anti-CD59 Antibody (APC)||Rabbit||Monoclonal||FCM||12474-R029-A|
|Anti-CD59 Antibody (PE)||Rabbit||Monoclonal||FCM||12474-R029-P|