CD59 Antibody

CD59 Antibody Datasheet

CD59 Antibody Product Information

• Immunogen: Recombinant Human CD59 protein (Catalog#12474-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 029
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD59 (rh CD59; Catalog#12474-H08H; P13987-1; Met 1-Glu 101).

CD59 Antibody Usage Guide

• Specificity: Human CD59
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD59. The detection limit for Human CD59 is approximately 0.0049 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD59 Antibody Background

CD59 glycoprotein, also known as 20 kDa homologous restriction factor, HRF20, MAC-inhibitory protein, Membrane attack complex inhibition factor, Membrane inhibitor of reactive lysis, MIC11, MIRL and CD59, is a cell membrane protein which contains oneUPAR/Ly6 domain. CD59 is a small, highly glycosylated, GPI-linked protein, with a wide expression profile. The soluble form of CD59 from urine retains its specific complement binding activity, but exhibits greatly reduced ability to inhibit MAC assembly on cell membranes. CD59 is a potent inhibitor of the complement membrane attack complex (MAC) action. CD59 was first identified as a regulator of the terminal pathway of complement. It acts by binding to the C8 and/or C9 complements of the assembling MAC, thereby preventing incorporation of the multiple copies of C9 required for complete formation of the osmolytic pore. This inhibitor appears to be species-specific. CD59 is involved in signal transduction for T-cell activation complexed to a protein tyrosine kinase. Defects in CD59 are the cause of CD59 deficiency (CD59D).

References

1. Fletcher C.M., et al., 1994, Structure 2: 185-199.
2. Rudd P.M., et al., 1997, J. Biol. Chem. 272: 7229-7244.
3. Kimberley,F.C. et al., 2007,Mol Immunol. 44 (1-3): 73-81.
4. Gong,Y. et al., 2007, Sci China C Life Sci. 50 (6): 773-9.
5. Picariello G., et al., 2008, Proteomics 8: 3833-3847.
6. Heibeck T.H., et al., 2009, J. Proteome Res. 8: 3852-3861.