CD55 / DAF Antibody

CD55 / DAF Antibody

CD55 / DAF Antibody Product Information

• Immunogen: Recombinant human CD55 protein ( Catalog#10101-H08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 028
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant human CD55 / DAF ( rh CD55 ; Catalog#10101-H08H ; NP_000565.1 ; Met 1 - Ser 353 ).

CD55 / DAF Antibody Usage Guide

• Specificity: Human CD55 / DAF
• No cross-reactivity in ELISA with
  

  Human CD97

• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect human CD55 in WB. Using a DAB detection system, the detection limit for human CD55 is approximately 5 ng/lane under non-reducing conditions. Use of this antibody under reducing conditions is not recommended
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect human CD55. The detection limit for human CD55 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD55 / DAF Antibody Background

CD55, also well known as decay-accelerating factor (DAF), is a member of the RCA (regulators of complement activation) family characterized by four to 30 SCRs (short consensus repeats) in their plasma-exposed regions. This 70-75 kDa glycoprotein CD55 containing four SCR modules is involved in the regulation of the complement cascade. It interferes with the functions of C3 and C5 convertases in both the classical and alternative pathways by preventing the assembly of the C3bBb complex or accelerating the disassembly of preformed convertase, and thus disrupts the cascade and protects the host cells from damage. Antigens present on the CD55/DAF constitute determinant for the Cromer blood group system. CD55/DAF has been identified as a ligand for an activation-associated, seven-transmembrane lymphocyte receptor, CD97, which is a receptor mediating attachment and infection of several viruses and bacteria. In addition, it has been shown that CD55/DAF regulates the interplay between complement and T cell immunity in vivo, and thus may be implicated in immune and tumor biology. There are two variants of CD55 encoded by alternatively spliced transcripts, the predominant variant is a GPI-anchored membrane form ubiquitously expressed on cells exposed to plasma complement proteins, whereas a soluble form present at much lower level.

References

1. Bergelson, JM. et al., 1994, Proc. Natl. Acad. Sci. USA. 91: 6245-8.
2. Terstappen, LW. et al., 1992, J. Leuro. Biol. 52: 652-60.
3. Liu, J. et al., 2005, J. Exp. Med. 201: 567-77.
4. Madjd, Z. et al., 2004, Clin. Cancer. Res. 10: 2797-803.
5. Mikesch, JH. et al., 2007, Cell. Oncol. 28: 223-32.