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Your Position: Home > Gene Products > Other Species > Cynomolgus monkey CD53 Gene cDNA Clone / ORF Clone

Cynomolgus monkey CD53 Gene cDNA Clone / ORF Clone

Catalog	Vector (Price)	Quantity	In Stock	Operation
CG90189-G	Cloning vector ($95) pCMV / hygro ($295) pCMV / hygro - HA ($295) pCMV / hygro - His ($295) pCMV / hygro - Myc ($295) pCMV / hygro - FLAG ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD53 cDNA Clone Datasheet

CD53 cDNA Clone Product Information

cDNA Size:	660
Gene Synonym:	CD53
Source:	Cynomolgus monkey

CD53 cDNA Clone Description

cDNA Description:	ORF Clone of Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey) CD53 molecule DNA.
Vector:	Cloning Vector: pGEM-T Vector Expression Vector: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutations: 301 T/A resulting in the amino acid Phe substitution by Ile and 555 C/T not causing the amino acid variation. Please check the sequence information before order.

CD53 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD53 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD53 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGGGCATGA GTAGCTTGAA ATTGCTGAAG TATGTCCTGT TTTTCTTCAA CTTGCTCTTT
61       TGGATCTGTG GCTGCTGCAT TTTGGGCTTT GGGATCTACC TGCTGATCCA CAACAACTTC
121      GGAGTGCTCT TCCATAACCT CCCCTCCCTC ACGCTGGGCA ATGTGTTTGT CATTGTGGGC
181      TCCATTATCA TGGTAGTTGC CTTCCTGGGC TGCATGGGCT CTATCAAGGA AAACAAGTGC
241      CTGCTTATGT CGTTCTTCAT CCTGCTGCTG ATTATCCTCC TTGCCGAGGT GATCTTGGCC
301      ATCCTGCTCT TTGTATATGA ACAGAAGCTG AATGAGTATG TGGCTAAGGG TCTGACCGAC
361      AGCATCCACC GTTACCACTC AGACAATAGC ACCAAGGCAG CGTGGGACTC CATCCAGTCA
421      TTTCTCCAGT GCTGTGGTAT AAATGGCACG AGTGATTGGA CCAGTGGCCC ACCAGCATCT
481      TGCCCCTCAG ATCCAAATGT GGAGGGTTGC TACGCGAAAG CAAGACTGTG GTTTCATTCC
541      AATTTCCTGT ATATTGGAAT CATCACCATC TGTGTATGTG TGATCGAGGT GTTGGGGATG
601      TCCTTTGCAC TGACCCTGAA CTGCCAGATT GATAAAACCA GTCAGAGCAT AGGGCTATGA

Quality Control:cDNA sequencing results is available upon request.