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Rat CD53 Gene cDNA Clone / ORF Clone
| Catalog | Vector (Price) | Quantity | In Stock | Operation |
| RG80303-G |
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YES |  |
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* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. |
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CD53 cDNA Clone Datasheet
CD53 cDNA Clone Product Information
| Gene Bank Ref.ID: | NM_012523.1 |
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cDNA Size: |
660 |
| Gene Synonym: |
OX44; Ox-44; RATOX44; Cd53 |
| Source: | Rat |
CD53 cDNA Clone Description
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cDNA Description: |
ORF Clone of Rattus norvegicus Cd53 molecule DNA. |
| Vector: | Cloning Vector: pGEM-T Vector
Expression Vectors: pCMV/hygro, pCMV/hygro-Myc, pCMV/hygro-His, pCMV/hygro-HA, pCMV/hygro-FLAG |
| Sequence Description: | Identical with the Gene Bank Ref. ID sequence except for the point mutation 102 T/C not causing the amino acid variation. |
CD53 cDNA Clone Shipping
- 5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
- It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.
CD53 cDNA Clone Usage Protocol
- Cut along the circle edge of the spot (arrow points to) to get the filter paper.
- Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H2O Ensure the filter is completely immersed in the H2O by briefly centrifuging the tube for 10 seconds.
- Transfer the tube to incubation at 95℃ for 15-30 min.
- At the end of the incubation pulse vortex, or gently tap the tube.
- Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.
CD53 cDNA Clone Sequence and Quality Control
Sequence:
1 ATGGGCATGA GCAGCCTGAA ATTGCTGAAA TATGTTCTGT TTTTCTTTAA CTTTCTTTTT 61 TGGGTCTGTG GCTGTTGCAT TTTGGGCTTT GGGATCCACC TCCTGGTCCA GAATACCTAT 121 GGAATACTCT TCCGTAATCT CCCCTTCCTG ACACTTGGCA ATGTTCTGGT CATTGTGGGC 181 TCCATTATCA TGGTAGTTGC CTTCTTGGGC TGCATGGGAT CAATCAAGGA GAATAAGTGC 241 CTGCTTATGT CGTTCTTTGT TCTGCTGCTG CTTATTCTCC TTGCTGAGGT GACCTTAGCC 301 ATCCTGCTCT TTGTGTATGA AAAAAAGATC AACACGTTAG TGGCAGAGGG CCTGAATGAC 361 AGCATCCAAC ATTATCACTC TGACAACAGC ACCAGGATGG CATGGGACTT CATCCAGTCA 421 CAACTGCAGT GTTGTGGTGT AAATGGCTCA AGTGATTGGA TCAGTGGTCC ACCATCTTCC 481 TGCCCATCAG GTGCAGATGT TCAGGGTTGT TATAAAAAAG GGCAGGCGTG GTTTCACTCC 541 AATTTCTTGT ATATTGGAAT CGTTACCATC TGCGTATGTG TGATACAGGT GCTGGGGATG 601 TCCTTTGCTC TGACACTGAA CTGCCAGATT GACAAAACCA GCCAGGCTTT AGGGCTGTGA
Quality Control:cDNA sequencing results is available upon request.
