CD50 Protein & Antibody (ICAM-3)

Cluster of Differentiation 50 (Intercellular Adhesion Molecule 3)

CD50 Products

Protein, Recombinant

Molecule	Species	Description	Cat. No
CD50/ICAM-3	Human	CD50/ICAM-3/Fc Protein, Recombinant	10333-H03H
CD50/ICAM-3	Human	CD50/ICAM-3 Protein, Recombinant	10333-H08H

  10333-H03H:  When cells are added to ICAM3 coated plates (12.5 µg/mL, 100 µl/well), approximately 45%-60% will adhere specifically. Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.

  10333-H08H:  When cells are added to ICAM3 coated plates (12.5 µg/mL, 100 µl/well), approximately 5%-15% will adhere specifically. Measured by the ability of the immobilized protein to support the adhesion of PMA stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells.

CD50 Antibody Products

Molecule	Application	Description	Cat. No
Human CD50/ICAM-3	WB, FCM	CD50 / ICAM-3 Antibody ( FITC )	10333-MM02-F
Human CD50/ICAM-3	WB, ELISA	CD50/ICAM-3 Antibody, Mouse MAb	10333-MM07
Human CD50/ICAM-3	WB, ELISA	CD50 / ICAM-3 Antibody	10333-RP01
Human CD50/ICAM-3	WB, ELISA	CD50 / ICAM-3 Antibody (Antigen Affinity Purified)	10333-RP02

CD50 ELISA Kit

Molecule	Application	Description	Cat. No
Human CD50/ICAM-3	ELISA	CD50/ICAM-3 ELISA Kit	SEK10333

CD50 cDNA Clone

Molecule	Species	Description	Cat. No
CD50/ICAM-3	Human	Homo sapiens CD50/ICAM-3 cDNA Clone(NM_002162.3)	HG10333-M

CD50 Alternative Names

CD50, ICAM-3, ICAM3, CDW50, ICAM-R

CD50 Related Areas

Immunology>>Adhesion Molecule>> Cell Adhesion Molecule (IgSF CAM) >>CD50/ICAM-3

Immunology>>Cluster of Differentiation>>Dendritic Cell CD Antigen>>Dendritic Cell Adhesion>>CD50/ICAM-3

CD50 Background

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

CD50 Related Studies

1. Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.
2. van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.
3. Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.
4. Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.