Anti-CD50 Antibody

Cat: 10333-RP02
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Anti-CD50 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD50 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD50
Recombinant Human ICAM3 / CD50 protein (Catalog#10333-H08H)
Produced in rabbits immunized with purified, recombinant Human ICAM3 / CD50 (rh ICAM3 / CD50; Catalog#10333-H08H; NP_002153.2; Met 1-His 485). ICAM3 / CD50 specific IgG was purified by Human ICAM3 / CD50 affinity chromatography.
Polyclonal Rabbit IgG
Protein A & Antigen Affinity
0.2 μm filtered solution in PBS with 5% trehalose
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD50 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:2000  
ELISA 1:5000-1:10000  
IP 4-6 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD50 Antibody (Rabbit Polyclonal antibody) Images
Human ICAM-3 / CD50 Immunoprecipitation(IP) 7974

ICAM3 was immunoprecipitated using:

Lane A:0.5 mg THP-1 Whole Cell Lysate

2 µL anti-ICAM3 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-ICAM3 rabbit polyclonal antibody,at 1:200 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 60 kDa

Observed band size: 170 kDa

Human ICAM-3 / CD50 Western blot (WB) 13187

Anti-ICAM3 rabbit polyclonal antibody at 1:500 dilution

Lane A: HL60 Membrane Lysate

Lysates/proteins at 20 μg per lane.


Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:60 kDa

Observed band size:150 kDa

Anti-CD50 Antibody Alternative Names
Anti-CD50 Antibody;Anti-CDW50 Antibody;Anti-ICAM-3 Antibody;Anti-ICAM-R Antibody
CD50 Background Information

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

Full Name
intercellular adhesion molecule 3
  • Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.
  • van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.
  • Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.
  • Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD50 Antibody (PE) Mouse Monoclonal FCM 10333-MM02-P
    Anti-CD50 Antibody Rabbit Monoclonal ELISA 10333-R101
    Anti-CD50 Antibody Mouse Monoclonal ELISA(Cap),FCM 10333-MM02
    Anti-CD50 Antibody Rabbit Monoclonal IHC-P 10333-R003
    Anti-CD50 Antibody (FITC) Mouse Monoclonal FCM 10333-MM02-F
    Anti-CD50 Antibody Mouse Monoclonal ELISA 10333-MM07
    Anti-CD50 Antibody Rabbit Polyclonal ELISA 10333-RP01
    Anti-CD50 Antibody Mouse Monoclonal IHC-P 10333-MM01
    Anti-CD50 Antibody (APC) Mouse Monoclonal FCM 10333-MM02-A
    Anti-CD50 Antibody Rabbit Monoclonal IHC-P 10333-R002
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