Anti-CD50 Antibody

Cat: 10333-MM07
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Anti-CD50 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD50 Antibody
Validated applications
ELISA
Species reactivity
Reacts with: Human
Specificity
Human CD50
No cross-reactivity in ELISA with Human ICAM-2 / CD102 Human CD106 / VCAM1 Mouse CD106 / VCAM1 Mouse ICAM-1 / CD54
Immunogen
Recombinant Human ICAM3 protein (Catalog#10333-H08H)
Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human ICAM3 (rh ICAM3; Catalog#10333-H08H; NP_002153.2; Met 1-His 485). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Source
Monoclonal Mouse IgG1 Clone #3B10B5B9
Purification
Protein A
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD50 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
ELISA 1:1000-1:2000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD50 Antibody Alternative Names
Anti-CD50 Antibody;Anti-CDW50 Antibody;Anti-ICAM-3 Antibody;Anti-ICAM-R Antibody
CD50 Background Information

The protein ICAM-3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. It is a DC-SIGN ligand that is constitutively expressed on resting leukocytes, and is thus an important molecule for the first immune response. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule. ICAM-3 binds to LFA-1 on antigen-presenting cells (APC) stabilizing the T cell-APC interaction, facilitating signaling through the CD3/TCR complex. However, recent evidence using cultured and transformed T cells suggests ICAM-3 may also function in signaling. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells. ICAM-3 is associated with an increase of cellular radio-resistance and cancer cell proliferation. It could be considered as a candidate for anti-cancer drug development and as a cancer diagnostic marker.

Full Name
intercellular adhesion molecule 3
References
  • Berney SM, et al. (1999) ICAM-3 (CD50) cross-linking augments signaling in CD3-activated peripheral human T lymphocytes. J Leukoc Biol. 65(6): 867-74.
  • van Buul JD, et al. (2004) ICAM-3 activation modulates cell-cell contacts of human bone marrow endothelial cells. J Vasc Res. 41(1): 28-37.
  • Sugino H. (2005) ICAM-3, a ligand for DC-SIGN, was duplicated from ICAM-1 in mammalian evolution, but was lost in the rodent genome. FEBS Lett. 579(13): 2901-6.
  • Park JK, et al. (2010) ICAM-3 enhances the migratory and invasive potential of human non-small cell lung cancer cells by inducing MMP-2 and MMP-9 via Akt and CREB. Int J Oncol. 36(1): 181-92.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD50 Antibody (PE) Mouse Monoclonal FCM 10333-MM02-P
    Anti-CD50 Antibody Rabbit Monoclonal ELISA 10333-R101
    Anti-CD50 Antibody Rabbit Polyclonal WB,ELISA,IP 10333-RP02
    Anti-CD50 Antibody Mouse Monoclonal ELISA(Cap),FCM 10333-MM02
    Anti-CD50 Antibody Rabbit Monoclonal IHC-P 10333-R003
    Anti-CD50 Antibody (FITC) Mouse Monoclonal FCM 10333-MM02-F
    Anti-CD50 Antibody Rabbit Monoclonal IHC-P 10333-R002
    Anti-CD50 Antibody Rabbit Polyclonal ELISA 10333-RP01
    Anti-CD50 Antibody (APC) Mouse Monoclonal FCM 10333-MM02-A
    Anti-CD50 Antibody Mouse Monoclonal IHC-P 10333-MM01
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