CD50 / ICAM-3 Antibody

CD50 / ICAM-3 Antibody Datasheet

CD50 / ICAM-3 Antibody Product Information

• Immunogen: Recombinant Human CD50 / ICAM-3 protein (Catalog#10333-H08H)
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 101
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD50 / ICAM-3 (rh CD50 / ICAM-3; Catalog#10333-H08H; NP_002153.2; Met 1-His 485).

CD50 / ICAM-3 Antibody Usage Guide

• Specificity: Human CD50 / ICAM-3
• No cross-reactivity in ELISA with

  Human ICAM2

  Human VCAM1

  Mouse ICAM1

• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human ICAM3. The detection limit for Human ICAM3 is approximately 0.00245 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD50 / ICAM-3 Antibody Background

The protein ICAM3, also known as CD50, is a member of the intercellular adhesion molecule (ICAM) family consisting three members. All ICAM proteins are type I transmembrane glycoproteins, and are ligands for leukocyte function-associated antigen 1 (LFA-1) expressed on the surface of all white blood cells. ICAM-3 comprises of five immunoglobulin-like domains, and binds LFA-1 through its two N-terminal domains. It functions not only as an adhesion molecule, but also as a potent signalling molecule.ICAM3 is constitutively and abundantly expressed by all leucocytes, and thus is the major ligand for LFA-1 in the initiation of primary immune responses. It has been reported that CD50 molecule can play a role in developing functionally mature T lymphocytes and its expression increases during the maturation process of T lymphocytes. In addition, the interactions of ICAM-3 and LFA-1 facilitate HIV-1- induced virological synapse formation between T cells.

References

1. Fawcett, J. et al., 1992, Nature, 360: 481-484.
2. Pino-otin, M.R. et al., 1995, Tissue. Antigens. 46: 32-44.
3. Simmons, D.L. et al., 1995, Cancer. Surv. 24: 141-155.
4. Jolly, C. et al., 2007, J. Virol. 81: 13916-13921.