|Datasheet||Specific References||Reviews||Related Products||Protocols|
|ORF Clone of Rattus norvegicus Cd5 molecule DNA.|
|Identical with the Gene Bank Ref. ID sequence.|
|Whatman FTA elute card (Cat: WB120410) contains 5-10 μg of plasmid.|
|The Whatman FTA elute card can be stored at room temperature for three months under dry condition.|
The pGEM-T is 3kb in length, and contains the amplicin resistance gene, conferring selection of the plasmid in E. coli, and the ori site which is the bacterial origin of replication. The plasmid has multiple cloning sites as shown below. The coding sequence was inserted by TA cloning. Many E. coli strains are suitable for the propagation of this vector including JM109, DH5α and TOP10.
The coding sequence can be easily obtained by digesting the vector with proper restriction enzyme(s). The coding sequence can also be amplified by PCR with M13 primers, or primer pair SP6 and T7.
|Rat CD5 Gene cDNA Clone (full-length ORF Clone), expression ready, FLAG-tagged||RG80374-G-F|
|Rat CD5 Gene cDNA Clone (full-length ORF Clone), expression ready, His-tagged||RG80374-G-H|
|Rat CD5 Gene cDNA Clone (full-length ORF Clone), expression ready, Myc-tagged||RG80374-G-M|
|Rat CD5 Gene cDNA Clone (full-length ORF Clone), expression ready, untagged||RG80374-G-N|
|Rat CD5 Gene cDNA Clone (full-length ORF Clone), expression ready, HA-tagged||RG80374-G-Y|
|Product name||Product name|
The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.