Rat CD5 Protein

Cd5 molecule Protein Datasheet

CD5 Protein Product Information

• Synonym: CD5
• Protein Construction: A DNA sequence encoding the rat CD5 (P51882) (Met1-Pro368) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD5 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Gly 24
• Molecular Mass: The recombinant rat CD5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 586 amino acids and has a predicted molecular mass of 64.5 kDa. The apparent molecular mass of the protein is approximately 85 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD5 protein

CD5 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD5 Protein Description

T-cell surface glycoprotein CD5, also known as Lymphocyte antigen T1 and LEU1, is a single-pass type I  membrane protein. CD5 contains 3 SRCR domains and has been regarded as a possible B cell lineage marker differentiating subsets, CD5+ B cells (also termed B1 cells) and conventional B cells (or B2 cells). CD5 is expressed at various developmental and activation stages on human B cells. It is a well established negative regulator of TCR and BCR signalling. Increased expression of CD5 on either T cells or B cells protects against autoimmunity. CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. The physiological role of CD5 might be to control the generation of aberrant immune responses. In cancers, CD5 expression plays a role in the fate of tumour-specific T cells, rendering lymphocytes tolerant and unable to recognize and eliminate malignant cells. Development, selection and function of different B- and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules.

References

1. Heuring AH. et al., 2001, The British journal of ophthalmology. 85 (4): 498-9.
2. Dalloul A. 2009, Autoimmun Rev. 8 (4): 349-53.
3. Pospisil R. et al., 2009, Mol Immunol. 46 (13): 2456-64.