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Product Catalog
CD5 ELISA Pair Set, Mouse ELISA Pair Set 
| Catalog | Size (Price) | Quantity | In Stock | Operation | Other Information |
| SEK50403 |
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Mouse CD5 ELISA Pair Set
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Order or Inquire for CD5 ELISA Pair Set |
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High affinity Mouse CD5 ELISA Pair Set |
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Detection limit - 12.5 pg/ml |
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Affordable price and 30%-80% cost saving for Bulk order |
- Materials
- Protocol
- Trouble Shooting
- Image & Flash
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No signal |
Incorrect or no Detection Antibody was added |
Add appropriate Detection Antibody and continue |
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Substrate solution was not added |
Add substrate solution and continue |
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Incorrect storage condition |
Check if the kit is stored at recommended condition and used before expiration date |
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Poor Standard Curve |
Standard was incompletely reconstituted or was inappropriately stored |
Aliquot reconstituted standard and store at -70 ℃ |
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Imprecise / inaccurate pipetting |
Check / calibrate pipettes |
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Incubations done at inappropriate temperature, timing or agitation |
Follow the general ELISA protocol |
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Background wells were contaminated |
Avoid cross contamination by using the sealer appropriately |
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Poor detection value |
The concentration of antigen in samples was too low |
Enriching samples to increase the concentration of antigen |
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Samples were ineffective |
Check if the samples are stored at cold environment. Detect samples in timely manner |
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High Background |
Insufficient washes |
Use multichannel pipettes without touching the reagents on the plate |
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Increase cycles of washes and soaking time between washes |
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TMB Substrate Solution was contaminated |
TMB Substrate Solution should be clear and colorless prior to addition to wells |
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Materials were contaminated |
Use clean plates, tubes and pipettes tips |
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Non-specificity |
Samples were contaminated |
Avoid cross contamination of samples |
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The concentration of samples was too high |
Try higher dilution rate of samples |
Problems 
Possible Sources 
Solutions 
Mouse CD5 ELISA Pair Set Related Products & Topics
Related Areas:
Proteins:
Antibodies:
Background
T-cell surface glycoprotein CD5, also known as Lymphocyte antigen T1 and LEU1, is a single-pass type I membrane protein. CD5 contains 3 SRCR domains and has been regarded as a possible B cell lineage marker differentiating subsets, CD5+ B cells (also termed B1 cells) and conventional B cells (or B2 cells). CD5 is expressed at various developmental and activation stages on human B cells. It is a well established negative regulator of TCR and BCR signalling. Increased expression of CD5 on either T cells or B cells protects against autoimmunity. CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. The physiological role of CD5 might be to control the generation of aberrant immune responses. In cancers, CD5 expression plays a role in the fate of tumour-specific T cells, rendering lymphocytes tolerant and unable to recognize and eliminate malignant cells. Development, selection and function of different B-cell and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules. CD5(+) B cells increase physiologically during mouse adolescence, and subsequently decrease with aging.
