CD5 / LEU1 Antibody

Mouse T-cell Surface Glycoprotein CD5 Antibody

CD5 / LEU1 Antibody Product Information

• Immunogen: Recombinant mouse CD5 protein ( Catalog# 50403-M08H )
• Antibody Type: Rabbit Monoclonal Antibody ( Rabbit mAb Service Platform )
• Clone ID: 105
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was obtained from a rabbit immunized with purified, human cell-derived, recombinant mouse CD5 extracellular domain ( rMCD5 ; Catalog#50403-M08H ; NP_031676.3 ; Met 1 - Pro 371 ).

CD5 / LEU1 Antibody Usage Guide

• Specificity: Mouse CD5 / LEU1
• Western blot: This antibody can be used at 1 - 2 μg/mL with the appropriate secondary reagents to detect mouse CD5 in WB. Using a DAB detection system, the detection limit for mouse CD5 is approximately 0.5 ng/lane under non-reducing conditions
• Direct ELISA: This antibody can be used at 0.1 - 0.2 μg/mL with the appropriate secondary reagents to detect mouse CD5. The detection limit for mouse CD5 is approximately 0.00975 ng/well
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD5 / LEU1 Antibody Background

Mouse T-cell surface glycoprotein CD5, also known as Lymphocyte antigen T1 and LEU1, is a single-pass type I membrane protein. CD5 contains 3 SRCR domains and has been regarded as a possible B cell lineage marker differentiating subsets, CD5+ B cells (also termed B1 cells) and conventional B cells (or B2 cells). CD5 is expressed at various developmental and activation stages on human B cells. It is a well established negative regulator of TCR and BCR signalling. Increased expression of CD5 on either T cells or B cells protects against autoimmunity. CD5-positive cells may also prevent the emergence of autoimmunity by provision of cytokines such as IL-10. The physiological role of CD5 might be to control the generation of aberrant immune responses. In cancers, CD5 expression plays a role in the fate of tumour-specific T cells, rendering lymphocytes tolerant and unable to recognize and eliminate malignant cells. Development, selection and function of different B-cell and T-cell subsets or their preferential survival may be directly or indirectly dependent on different glycan structures associated with CD5 or CD5-like molecules. CD5(+) B cells increase physiologically during mouse adolescence, and subsequently decrease with aging.

References

1. Watanabe, K et al., 2001, Mech Ageing Dev. 122 (15):1787-95.
2. Dalloul, A., 2009, Autoimmun Rev. 8 (4): 349-53.
3. Pospisil, R. et al., 2009, Mol Immunol. 46 (13): 2456-64.
4. Heuring, AH. et al., 2001, The British journal of ophthalmology. 85 (4): 498-9.