Anti-CD5 Antibody

Cat: 50403-RP02
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Anti-CD5 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD5 Antibody
Validated applications
WB,ELISA,IHC-P
Species reactivity
Reacts with: Mouse, Rat
Not React with: Human
Specificity
Mouse CD5
Immunogen
Recombinant Mouse CD5 protein (Catalog#50403-M08H)
Preparation
Produced in rabbits immunized with purified, recombinant Mouse CD5 extracellular domain (rM CD5; Catalog#50403-M08H; NP_031676.3; Met 1-Pro 371). CD5 specific IgG was purified by mouse CD5 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD5 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IHC-P 1:10000-1:50000  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD5 Antibody (Rabbit Polyclonal antibody) Images
Mouse CD5 / Cluster of Differentiation 5 Immunohistochemistry(IHC) 3341
Immunochemical staining of mouse CD5 in mouse spleen with rabbit polyclonal antibody (1:25000, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of T cells in periarterial lymphatic sheath.
Mouse CD5 / Cluster of Differentiation 5 Western blot (WB) 13671

Anti-CD59 rabbit polyclonal antibody at 1:500 dilution

Lane A: Jurkat Whole Cell Lysate

Lane B: Hu-T78 Whole Cell Lysate

Lysates/proteins at 30 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:55 kDa

Observed band size:90 kDa

Anti-CD5 Antibody Alternative Names
Anti-Ly-1 Antibody;Anti-Ly-12 Antibody;Anti-Ly-A Antibody;Anti-Lyt-1 Antibody
CD5 Background Information

The cluster of differentiation (CD) system is commonly used as cell markers in immunophynotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 320 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. CD5 is a member of the CD system. CD5 was found to be widely distributed in T-cells and B1 cells which is a subset of IgM-secreting B cells. CD5 also was found expressed in small lymphocytic lymphoma, hairy cell leukaemia and mantle cell lymphoma cells. CD5 serves to weaken the activating stimulus from the BCR so that the B1 cells can only reflect to the very strong stimuli but not the normal tissue proteins.

Full Name
CD5 molecule
References
  • Zola H, et al. (2007) CD molecules 2006-human cell differentiation molecules. J Immunol Methods. 318 (1-2): 1-5.
  • Ho IC, et al. (2009) GATA3 and the T-cell lineage: essential functions before and after T-helper-2-cell differentiation. Nat Rev Immunol. 9 (2): 125-35.
  • Matesanz-Isabel J, et al. (2011) New B-cell CD molecules. Immunology Letters. 134 (2): 104-12.
  • Kirchgessner H, et al. (2001) The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 193 (11): 1269-84.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD5 Antibody Mouse Monoclonal FCM 80374-MM01
    Anti-CD5 Antibody Rabbit Monoclonal ELISA 80374-R006
    Anti-CD5 Antibody Mouse Monoclonal FCM 80374-MM06
    Anti-CD5 Antibody (APC) Mouse Monoclonal FCM 80374-MM06-A
    Anti-CD5 Antibody (PE) Mouse Monoclonal FCM 80374-MM06-P
    Anti-CD5 Antibody Mouse Monoclonal ELISA 80374-MM03
    Anti-CD5 Antibody (FITC) Mouse Monoclonal FCM 80374-MM06-F
    Anti-CD5 Antibody (FITC) Mouse Monoclonal FCM 80374-MM01-F
    Anti-CD5 Antibody Rabbit Polyclonal ELISA 80374-RP01
    Anti-CD5 Antibody Rabbit Polyclonal ELISA,IHC-P 80374-T24
    Anti-CD5 Antibody Rabbit Polyclonal ELISA 50403-RP01
    Anti-CD5 Antibody Rabbit Monoclonal FCM,ICC/IF,IF 50403-R114
    Anti-CD5 Antibody (PerCP) Rabbit Monoclonal FCM 50403-R249-C
    Anti-CD5 Antibody Rabbit Monoclonal ELISA(Cap) 50403-R101
    Anti-CD5 Antibody Rabbit Monoclonal ELISA 50403-R105
    Anti-CD5 Antibody (APC) Rabbit Monoclonal FCM 50403-R114-A
    Anti-CD5 Antibody (PerCP) Rabbit Monoclonal FCM 50403-R114-C
    Anti-CD5 Antibody (PE) Rabbit Monoclonal FCM 50403-R114-P
    Anti-CD5 Antibody Rabbit Monoclonal FCM 50403-R249
    Anti-CD5 Antibody (FITC) Rabbit Monoclonal FCM 50403-R249-F
    Anti-CD5 Antibody (PE) Rabbit Monoclonal FCM 50403-R249-P
    Anti-CD5 Antibody (APC) Rabbit Monoclonal FCM 50403-R249-A
    Anti-CD5 Antibody (FITC) Rabbit Monoclonal FCM 50403-R114-F
    Anti-CD5 Antibody Rabbit Monoclonal ELISA 11027-R322
    Anti-CD5 Antibody (PE) Rabbit Monoclonal FCM 11027-R028-P
    Anti-CD5 Antibody (FITC) Rabbit Monoclonal FCM 11027-R039-F
    Anti-CD5 Antibody Rabbit Monoclonal FCM 11027-R028
    Anti-CD5 Antibody Rabbit Monoclonal ELISA,FCM 11027-R039
    Anti-CD5 Antibody Rabbit Polyclonal WB,ELISA,IHC-P 11027-T56
    Anti-CD5 Antibody Mouse Monoclonal ELISA 11027-MM05
    Anti-CD5 Antibody (APC) Rabbit Monoclonal FCM 11027-R028-A
    Anti-CD5 Antibody (PerCP) Rabbit Monoclonal FCM 11027-R028-C
    Anti-CD5 Antibody (PerCP) Rabbit Monoclonal FCM 11027-R039-C
    Anti-CD5 Antibody Rabbit Monoclonal WB 11027-R505
    Anti-CD5 Antibody (PE) Rabbit Monoclonal FCM 11027-R039-P
    Anti-CD5 Antibody (FITC) Rabbit Monoclonal FCM 11027-R028-F
    Anti-CD5 Antibody Rabbit Polyclonal WB,ELISA 11027-RP01
    Anti-CD5 Antibody (APC) Rabbit Monoclonal FCM 11027-R039-A
    Anti-CD5 Antibody Rabbit Polyclonal WB,ELISA,IHC-P,IP 11027-RP02
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