CD48 / SLAMF2 Antibody ( FITC )

CD48 / SLAMF2 Antibody Datasheet

CD48 / SLAMF2 Antibody Product Information

• Immunogen: Recombinant Human CD48 / SLAMF2 protein (Catalog#10797-H08H)
• Reagents: FITC-conjugated rabbit monoclonal antibody
• Clone ID: 248
• Ig Type: Rabbit IgG
• Concentration: 5 μl/Test, 0.1 mg/ml
• Formulation: Aqueous solution containing 0.5% BSA and 0.1% sodium azide
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human CD48 / SLAMF2 (rh CD48 / SLAMF2; Catalog#10797-H08H; NP_001769.2; Met 1-Ser 220) and conjugated with FITC under optimum conditions, the unreacted FITC was removed.

CD48 / SLAMF2 Antibody Usage Guide

• Specificity: Human CD48 / SLAMF2
• No cross-reactivity in ELISA with
  

  Mouse CD48

  Human TNFRSF4

• Flow Cytometry: Flow cytometric analysis of anti-CD48 reactivity.
  

  Profile of anti-CD48 reactivity on human peripheral blood lymphocytes analyzed by flow cytometry.

  Flow cytometry was performed on a BD FACSCalibur flow cytometry system

  Please refer to www.sinobiological.com/Flow-Cytometry-FACS-Protocols-a-750.html for technical protocols.

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD48 in WB.
• Storage: This antibody is stable for 12 months from date of receipt when stored at 2℃- 8℃. Protected from prolonged exposure to light. Do not freeze !
  Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.

CD48 / SLAMF2 Antibody Background

CD48, also known as SLAMF2, BCM-1 and BLAST-1, is a 65 kDa GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. In rodents, CD48 is the ligand for CD2 whereas in humans, CD58 is the ligand for CD2. Human CD48 cDNA encodes a 243 aa precursor that includes a 26 aa signal sequence, a 194 aa mature protein that contains one Ig-like V-type domain and one Ig-like C2-type domain, and a 23 aa C-terminal propeptide. It has a high degree of sequence homology to CD58 (LFA-3), and human CD48 shares approximately 50% aa sequence identity with mouse and rat CD48. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses.

References

1. Wong, Y.W. et al., 1990, J. Exp. Med. 171: 2115-2130.
2. Ianelli,C.J. et al., 1997,J Immunol. 159 (8): 3910-3920.
3. Ianelli, C.J. et al., 1998, J. Biol. Chem. 273: 23367-23375.
4. Loertscher, R. et al., 2002, Transpl. Immunol. 9: 93-96.
5. Assarsson, E. et al., 2005, J. Immunol. 175: 2045-2049.
6. Bhat, R. et al., 2006, J. Leukocyte Biol. 79: 417-424.