Mouse CD48 ORF Expression Clone, untagged

Catalog	Vector (Price)	Quantity	In Stock	Operation
MG50415-M-N	pCMV / hygro ($295)		YES
* pCMV vectors are ALL featured with a CMV promoter and a hygromycin-resistance (hygro) gene. These plasmids can be used directly in mammalian cell expression. Learn more about pCMV vectors. Notice: Additional handling time(2-3 weeks) may be required if a pCMV vector is selected.

CD48 cDNA Clone Datasheet

CD48 cDNA Clone Product Information

Gene Bank Ref.ID:	NM_007649.4
cDNA Size:	723
Gene Synonym:	BCM1, BLAST, Bcm-1, BLAST1, SLAMF2, Sgp-60, BLAST-1, MEM-102, AI449234, AW610730, Cd48
Source:	Mouse

CD48 cDNA Clone Description

cDNA Description:	ORF Clone of Mus musculus CD48 antigen DNA.
Vector:	pCMV/hygro
Sequence Description:	Identical with the Gene Bank Ref. ID sequence except for the point mutation 268 A/G resulting in the amino acid Asn substitution by Asp.

CD48 cDNA Clone Shipping

5-10μg Plasmid DNA is shipped on Whatman FTA elute card (Cat. No.: WB120410) at room temperature.
It would take 7-14 days for delivery typically. For bulk orders, additional 1-2 weeks would be needed.

CD48 cDNA Clone Usage Protocol

Cut along the circle edge of the spot (arrow points to) to get the filter paper.
Transfer the filter paper to a 1.5ml microfuge tube containing 150-200 μl of sterile H₂O Ensure the filter is completely immersed in the H₂O by briefly centrifuging the tube for 10 seconds.
Transfer the tube to incubation at 95℃ for 15-30 min.
At the end of the incubation pulse vortex, or gently tap the tube.
Briefly centrifuge for 30 seconds, to separate the matrix from the elute. The elute now contains the purified DNA.

CD48 cDNA Clone Sequence and Quality Control

Sequence:

1        ATGTGCTTCA TAAAACAGGG ATGGTGTCTG GTCCTGGAAC TGCTACTGCT GCCCTTGGGA
61       ACTGGATTTC AAGGTCATTC AATACCAGAT ATAAATGCCA CCACCGGCAG CAATGTAACC
121      CTGAAAATCC ATAAGGACCC ACTTGGACCA TATAAACGTA TCACCTGGCT TCATACTAAA
181      AATCAGAAGA TTTTAGAGTA CAACTATAAT AGTACAAAGA CAATCTTCGA GTCTGAATTT
241      AAAGGCAGGG TTTATCTTGA AGAAAACGAT GGTGCACTTC ATATCTCTAA TGTCCGGAAA
301      GAGGACAAAG GTACCTACTA CATGAGAGTG CTGCGTGAAA CTGAGAACGA GTTGAAGATA
361      ACCCTGGAAG TATTTGATCC TGTGCCCAAG CCTTCCATAG AAATCAATAA GACTGAAGCG
421      TCGACTGATT CCTGTCACCT GAGGCTATCG TGTGAGGTAA AGGACCAGCA TGTTGACTAT
481      ACTTGGTATG AGAGCTCGGG ACCTTTCCCC AAAAAGAGTC CAGGATATGT GCTCGATCTC
541      ATCGTCACAC CACAGAACAA GTCTACATTT TACACCTGCC AAGTCAGCAA TCCTGTAAGC
601      AGCAAGAACG ACACAGTGTA CTTCACTCTA CCTTGTGATC TAGCCAGATC TTCTGGAGTA
661      TGTTGGACTG CAACTTGGCT AGTGGTCACA ACACTCATCA TTCACAGGAT CCTGTTAACC
721      TAA

Quality Control:cDNA sequencing results is available upon request.