Rat CD48 / BCM1 / SLAMF2 Protein

Cd48 molecule Protein Datasheet

CD48 / BCM1 / SLAMF2 Protein Product Information

• Synonym: CD48, Bcm-1
• Protein Construction: A DNA sequence encoding the rat CD48 (P10252) (Met1-Arg216) was expressed, fused with the Fc region of human IgG₁ at the C-terminus.
• Source: Rat
• Expression Host: Human Cells

CD48 / BCM1 / SLAMF2 Protein QC Testing

• Purity: > 95% as determined by SDS-PAGE
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Predicted N terminal: Phe 23
• Molecular Mass: The recombinant rat CD48/Fc is a disulfide-linked homodimer. The reduced monomer comprises 435 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 38-44 kDa in SDS-PAGE under reducing conditions.
• Formulation: Lyophilized from sterile PBS, pH7.4.
  1. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
  2. Please contact us for any concerns or special requirements.
• SDS-PAGE: CD48 / BCM1 / SLAMF2 protein

CD48 / BCM1 / SLAMF2 Protein Usage Guide

• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

CD48 / BCM1 / SLAMF2 Protein Description

CD48, also known as SLAMF2, BCM-1 and BLAST-1, is a GPI-linked protein belonging to the CD2 subfamily of immunoglobulin superfamily molecules. CD48 is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. CD48 has a striking array of biological functions, among them adhesion, pathogen recognition, cellular activation, and cytokine regulation. CD48 performs biological functions in a variety processes including adhesion, pathogen recognition, cellular activation, and cytokine regulation, and emerges as a critical effector molecule in immune responses. In rodents, CD48 is the ligand for CD2 whereas in humans, CD58 is the ligand for CD2. CD48 has a high degree of sequence homology to CD58 (LFA-3), and Mouse CD48 shares approximately 50% aa sequence identity with human CD48.

References

1. Wong YW. et al., 1990, J Exp Med. 171: 2115-30.
2. Ianelli CJ. et al., 1997, J Immunol. 159 (8): 3910-20.
3. Ianelli CJ. et al., 1998, J Biol Chem. 273: 23367-75.
4. Loertscher, R. et al., 2002, Transpl Immunol. 9: 93-6.
5. Assarsson E. et al., 2005, J Immunol. 175: 2045-9.
6. Bhat R. et al., 2006, J Leukocyte Biol. 79: 417-24.
7. Munitz A. et al., 2007, Recent Pat Inflamm Allergy Drug Discov. 1 (1): 9-12.