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CD46 Antibody ( Antigen Affinity Purified ) Datasheet
|Order or Inquire for CD46 Antibody product||Quality antibodies||Antibody production services|
|Detection limit is 0.5 ng/lane in WB|
|Detection limit is 0.0049 ng/well in ELISA|
CD46 Antibody Product Information
Recombinant Human CD46 protein (Catalog#12239-H08H)
|Antibody Type :||Rabbit Polyclonal Antibody ( Antibody Purification Platform )|
|Ig Type :||
|Formulation :||0.2 μm filtered solution in PBS with 5% trehalose|
Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.
CD46 Antibody Usage Guide
IHC-P: 0.2-1 μg/mL
|Direct ELISA :||This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD46. The detection limit for Human CD46 is approximately 0.0049 ng/well.|
|Storage :||This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
CD46 Antibody Related Products & Topics
CD46 Antibody Background
CD46 complement regulatory protein, also known as membrane cofactor protein, Trophoblast leukocyte common antigen, is a single-pass type I membrane protein which contains fourSushi (CCP/SCR) domains. CD46 is a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. CD46 is expressed by all cells except erythrocytes. CD46 acts as a cofactor for complement factor I. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) which is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea.
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- Wang G. et al., 2000, J. Immunol. 164: 1839-1846.
- Hawkins,E.D. et al., 2010, FEBS Lett. 584 (24): 4838-44.
- Maga T.K. et al., 2010, Hum. Mutat. 31: E1445-E1460.