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CD46 Antibody, Rabbit PAb, Antigen Affinity Purified

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Human CD46 Antibody Product Information
Immunogen:Recombinant Human CD46 protein (Catalog#12239-H08H)
Clone ID:
Ig Type:Rabbit IgG
Formulation:0.2 μm filtered solution in PBS with 5% trehalose
Preparation:Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.
Human CD46 Antibody Usage Guide
Specificity:Human CD46
Application:WB, ELISA, IHC-P, IP

WB: 10-20 μg/ml

ELISA: 0.1-0.2 μg/mL

This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD46. The detection limit for Human CD46 is approximately 0.0049 ng/well.

IHC-P: 0.2-1 μg/mL

IP: 4-6 μg/mg of lysate

Storage:This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Human CD46 Antibody WB Application Image
CD46 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
Human CD46 Antibody IHC Application Image
[Click to enlarge image]
Immunochemical staining of human CD46 in human placenta with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections). Positive staining was localized to membrane of trophoblast.
CD46 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Human CD46 Antibody IP Application Image
CD46 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
Other CD46 Antibody Products
CD46 Background

CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.

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Catalog: 12239-RP02-200
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