Anti-CD46 Antibody

Cat: 12239-RP02
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Anti-CD46 Antibody (Rabbit Polyclonal antibody) General Information
Product name
Anti-CD46 Antibody
Validated applications
WB,ELISA,IHC-P,IP
Species reactivity
Reacts with: Human
Specificity
Human CD46
Immunogen
Recombinant Human CD46 protein (Catalog#12239-H08H)
Preparation
Produced in rabbits immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). CD46 specific IgG was purified by Human CD46 affinity chromatography.
Source
Polyclonal Rabbit IgG
Purification
Protein A & Antigen Affinity
Formulation
0.2 μm filtered solution in PBS
Conjugate
Unconjugated
Form
Liquid
Shipping
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD46 Antibody (Rabbit Polyclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:25000-1:50000  
IHC-P 1:2500-1:15000  
IP 1-2 μL/mg of lysate  

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD46 Antibody (Rabbit Polyclonal antibody) Images
Human CD46 Immunohistochemistry(IHC) 4494
Immunochemical staining of human CD46 in human placenta with rabbit polyclonal antibody at 1:10000 dilution, formalin-fixed paraffin embedded sections. Positive staining was localized to membrane of trophoblast.
Human CD46 Western blot (WB) 6661

Anti-CD46 rabbit polyclonal antibody at 1:500 dilution

Lane A: Hela Membrane Lysate

Lysates/proteins at 20 μg per lane.

Secondary

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:44 kDa

Observed band size:62 kDa

Human CD46 Immunoprecipitation(IP) 17660

CD46 was immunoprecipitated using:

Lane A:0.5 mg 293T Whole Cell Lysate

0.5 µL anti-CD46 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD46 rabbit polyclonal antibody,at 1:1000 dilution

Secondary antibody:

Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 44 kDa

Observed band size: 62 kDa

Anti-CD46 Antibody Alternative Names
Anti-AHUS2 Antibody;Anti-MCP Antibody;Anti-MIC10 Antibody;Anti-TLX Antibody;Anti-TRA2.10 Antibody
CD46 Background Information

CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 50% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.

Full Name
CD46 molecule, complement regulatory protein
References
  • Lublin D.M., et al.,(1988), Molecular cloning and chromosomal localization of human membrane cofactor protein (MCP). Evidence for inclusion in the multigene family of complement-regulatory proteins. J. Exp. Med. 168:181-194.
  • Purcell D.F., et al., (1991), Alternatively spliced RNAs encode several isoforms of CD46 (MCP), a regulator of complement activation.Immunogenetics 33:335-344.
  • Post T.W., et al.,(1991), Membrane cofactor protein of the complement system: alternative splicing of serine/threonine/proline-rich exons and cytoplasmic tails produces multiple isoforms that correlate with protein phenotype.J. Exp. Med. 174:93-102.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD46 Antibody Rabbit Polyclonal ELISA 12239-RP01
    Anti-CD46 Antibody Mouse Monoclonal FCM,ICC/IF,IF 12239-MM05
    Anti-CD46 Antibody (PE) Mouse Monoclonal FCM 12239-MM05-P
    Anti-CD46 Antibody (APC) Mouse Monoclonal FCM 12239-MM05-A
    Anti-CD46 Antibody Rabbit Monoclonal ELISA,IHC-P,ICC/IF,IF 12239-R001
    Anti-CD46 Antibody (FITC) Mouse Monoclonal FCM 12239-MM05-F
    Anti-CD46 Antibody Mouse Monoclonal ELISA,IHC-P 12239-MM01
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