|IP||1-2 μL/mg of lysate|
**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********
Anti-CD46 rabbit polyclonal antibody at 1:500 dilution
Lane A: Hela Membrane LysateLysates/proteins at 20 μg per lane.
Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.
Performed under reducing conditions.Predicted band size:44 kDa
Observed band size:62 kDa
CD46 was immunoprecipitated using:
Lane A:0.5 mg 293T Whole Cell Lysate0.5 µL anti-CD46 rabbit polyclonal antibody and 15 μl of 50 % Protein G agarose.Primary antibody:
Anti-CD46 rabbit polyclonal antibody,at 1:1000 dilutionSecondary antibody:
Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.
Performed under reducing conditions.Predicted band size: 44 kDa
Observed band size: 62 kDa
CD46, also known as Membrane Cofactor Protein (MCP), is a complement regulatory protein. CD46 is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 is expressed in white blood cells, platelets, epithelial cells, and fibroblasts. Human CD46 shares 50% amino acid sequence identity with mouse and rat CD46. The importance of CD46 to complement regulation is underscored by the observation that genetic loss of CD46 leads to development of atypical hemolytic-uremic syndrome (aHUS), a disease characterized by uncontrolled complement activation. CD46 is implicated in the development and/or progression of selected cancer types.
|Product Description||Host||Clonality||Application||Catalog# (PDF)|
|Anti-CD46 Antibody (APC)||Mouse||Monoclonal||FCM||12239-MM05-A|
|Anti-CD46 Antibody (PE)||Mouse||Monoclonal||FCM||12239-MM05-P|
|Anti-CD46 Antibody (FITC)||Mouse||Monoclonal||FCM||12239-MM05-F|