CD46 Antibody

CD46 Antibody Datasheet

CD46 Antibody Product Information

• Immunogen: Recombinant Human CD46 protein (Catalog#12239-H08H)
• Antibody Type: Mouse Monoclonal Antibody ( Mouse mAb Service Platform )
• Clone ID: 5H5C5D1
• Ig Type: Mouse IgG1
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD46 (rh CD46; Catalog#12239-H08H; EAW93471.1; Met 1-Asp 328). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.

CD46 Antibody Usage Guide

• Specificity: Human CD46
• No cross-reactivity in ELISA with

  Human cell lysate (293 cell line)

• Western blot: This antibody can be used at 1-2 μg/mL with the appropriate secondary reagents to detect Human CD46 in WB. Using a DAB detection system, the detection limit for Human CD46 is approximately 8 ng/lane under non-reducing conditions and 1 ng/lane under reducing conditions.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD46 Antibody Background

CD46 complement regulatory protein, also known as membrane cofactor protein, Trophoblast leukocyte common antigen, is a single-pass type I membrane protein which contains fourSushi (CCP/SCR) domains. CD46 is a serine protease which protects autologous cells against complement-mediated injury by cleaving C3b and C4b deposited on host tissue. CD46 is expressed by all cells except erythrocytes. CD46 acts as a cofactor for complement factor I. It may be involved in the fusion of the spermatozoa with the oocyte during fertilization. CD46 also acts as a costimulatory factor for T-cells which induces the differentiation of CD4+ into T-regulatory 1 cells. T-regulatory 1 cells suppress immune responses by secreting interleukin-10, and therefore are thought to prevent autoimmunity. A number of viral and bacterial pathogens seem to exploit this property and directly induce an immunosuppressive phenotype in T-cells by binding to CD46. Defects in CD46 are a cause of susceptibility to hemolytic uremic syndrome atypical type 2 (AHUS2) which is a complex genetic disease characterized by microangiopathic hemolytic anemia, thrombocytopenia, renal failure and absence of episodes of enterocolitis and diarrhea.

References

1. Kaellstroem H. et al., 1997,Mol. Microbiol. 25: 639-647.
2. Liszewski M.K. et al., 1998, J. Immunol. 161: 3711-3718.
3. Kemper C. et al., 2003, Nature 421: 388-392.
4. Wang G. et al., 2000, J. Immunol. 164: 1839-1846.
5. Hawkins,E.D. et al., 2010, FEBS Lett. 584 (24): 4838-44.
6. Maga T.K. et al., 2010, Hum. Mutat. 31: E1445-E1460.