Anti-CD44 Antibody

Cat: 12211-MM10

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Anti-CD44 Antibody (Mouse Monoclonal antibody) General Information
Product name
Anti-CD44 Antibody
Validated applications
Species reactivity
Reacts with: Human
Human CD44
No cross-reactivity in ELISA with Human DMP1 Human cell lysate (293 cell line)
Recombinant Human CD44 protein (Catalog#12211-H08H)
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Monoclonal Mouse IgG1 Clone #3E8C3
Protein A
0.2 μm filtered solution in PBS
This antibody is shipped as liquid solution at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Anti-CD44 Antibody (Mouse Monoclonal antibody) Validated Applications
Application Dilution Notes
WB 1:500-1:1000  
ELISA 1:5000-1:10000  
IP 0.2-1 μL/mg of lysate  
ELISA(Det) 1:5000-1:50000 This antibody will detect Human CD44 in ELISA pair set (Catalog: # SEK12211). In a sandwich ELISA, it can be used as detection antibody when paired with (Catalog: # 12211-MM01).

**********Please Note: Optimal concentrations/dilutions should be determined by the end user.**********

Anti-CD44 Antibody (Mouse Monoclonal antibody) Images
Human CD44 Immunoprecipitation(IP) 6188

CD44 was immunoprecipitated using:

Lane A:0.5 mg Hela Whole Cell Lysate

0.5 µL anti-CD44 mouse monoclonal antibody and 15 μl of 50 % Protein G agarose.

Primary antibody:

Anti-CD44 mouse monoclonal antibody,at 1:500 dilution

Secondary antibody:

Dylight 800-labeled antibody to Mouse IgG (H+L), at 1:7500 dilution

Developed using the odssey technique.

Performed under reducing conditions.

Predicted band size: 82 kDa

Observed band size: 98 kDa

Human CD44 Western blot (WB) 13477

Anti-CD44 mouse monoclonal antibody at 1:500 dilution

Lane A: Hela Whole Cell Lysate

Lysates/proteins at 30 μg per lane.


Goat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.

Developed using the Odyssey technique.

Performed under reducing conditions.

Predicted band size:82 kDa

Observed band size:98 kDa

Anti-CD44 Antibody Alternative Names
Anti-CDW44 Antibody;Anti-CSPG8 Antibody;Anti-ECMR-III Antibody;Anti-HCELL Antibody;Anti-HUTCH-I Antibody;Anti-IN Antibody;Anti-LHR Antibody;Anti-MC56 Antibody;Anti-MDU2 Antibody;Anti-MDU3 Antibody;Anti-MIC4 Antibody;Anti-Pgp1 Antibody
CD44 Background Information

CD44 is a type I transmembrane protein and a member of the cartilage link protein family. It is involved in cell-cell and cell-matrix interactions and signal transduction. Several CD44 ligands have been identified. The most extensively characterized ligand for CD44 is hyaluronan, a component of the extracellular matrix. CD44 protein is expressed on the majority of immune cells. The binding of CD44 to hyaluronan is induced on T lymphocytes after activation by antigen and on monocytes after stimulation by inflammatory agents. Under inflammatory conditions, CD44 on endothelial cells presents hyaluronan to CD44 on activated T lymphocytes and mediates a rolling interaction under flow conditions. Perturbations of the hyaluronan-CD44 interaction at the plasma membrane by various antagonists result in attenuation of receptor tyrosine kinase and transporter activities and inhibition of tumor progression in vivo. CD44 is known to interact with the ezrin family (ERM family) members and form a complex that plays diverse roles within both normal and abnormal cells, particularly cancer cells. CD44 and ezrin and their respective complex have properties suggesting that they may be important in the process of tumour-endothelium interactions, cell migrations, cell adhesion, tumour progression and metastasis.

Full Name
CD44 molecule (Indian blood group)
  • Bajorath J. (2000) Molecular organization, structural features, and ligand binding characteristics of CD44, a highly variable cell surface glycoprotein with multiple functions. Proteins. 39(2): 103-11.
  • Johnson P, et al. (2000) A role for the cell adhesion molecule CD44 and sulfation in leukocyte-endothelial cell adhesion during an inflammatory response? Biochem Pharmacol. 59(5): 455-65.
  • Martin TA, et al. (2003) The role of the CD44/ezrin complex in cancer metastasis. Crit Rev Oncol Hematol. 46(2): 165-86.
  • Toole BP, et al. (2008) Hyaluronan, CD44 and Emmprin: partners in cancer cell chemoresistance. Drug Resist Updat. 11(3): 110-21.
  • Johnson P, et al. (2009) CD44 and its role in inflammation and inflammatory diseases. Inflamm Allergy Drug Targets. 8(3): 208-20.
  • Product Description Host Clonality Application Catalog# (PDF)
    Anti-CD44 Antibody Rabbit Polyclonal IHC-P 101972-T08
    Anti-CD44 Antibody Rabbit Polyclonal WB,ELISA 12211-RP02
    Anti-CD44 Antibody Rabbit Monoclonal ELISA 12211-R065
    Anti-CD44 Antibody Rabbit Polyclonal ELISA 12211-RP01
    Anti-CD44 Antibody (FITC) Mouse Monoclonal FCM 12211-MM02-F
    Anti-CD44 Antibody Rabbit Monoclonal ICC/IF,IF 12211-R001
    Anti-CD44 Antibody Mouse Monoclonal WB,ELISA,ELISA(Cap),IHC-P,IP 12211-MM01
    Anti-CD44 Antibody Mouse Monoclonal FCM,ICC/IF,IF 12211-MM02
    Anti-CD44 Antibody (APC) Mouse Monoclonal FCM 12211-MM02-A
    Anti-CD44 Antibody (PE) Mouse Monoclonal FCM 12211-MM02-P
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