CD44 Antibody (Antigen Affinity Purified)

CD44 Antibody ( Antigen Affinity Purified ) Datasheet

CD44 Antibody Product Information

• Immunogen: Recombinant Human CD44 protein (Catalog#12211-H08H)
• Antibody Type: Rabbit Polyclonal Antibody ( Antibody Purification Platform )
• Ig Type: Rabbit IgG
• Formulation: 0.2 μm filtered solution in PBS with 5% trehalose
• Preparation: Produced in rabbits immunized with purified, recombinant Human CD44 (rh CD44; Catalog#12211-H08H; P16070-1; Met 1-Pro 220). CD44 specific IgG was purified by Human CD44 affinity chromatography.

CD44 Antibody Usage Guide

• Specificity: Human CD44
• Western blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD44 in WB. Using a DAB detection system, the detection limit for Human CD44 is approximately 4 ng/lane under non-reducing conditions and 0.5 ng/lane under reducing conditions.
• Direct ELISA: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CD44. The detection limit for Human CD44 is approximately 0.00975 ng/well.
• Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.
  Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.

CD44 Antibody Background

CD44, short for Cluster of Differentiation 44, is an adhesion molecule of the hyaluronate receptor family. It is expressed in a large number of mammalian cell types. The adhesive function of CD44, like other molecules involved in inducible adhesion, is tightly regulated. Post-translational modifications, isoform expression, aggregation state, and protein associations all can affect the ligand binding properties of CD44, and these can vary depending on the cell type and the activation state of the cell. The principal ligand of CD44 is hyaluronan, and CD44 is involved in the metabolism of this compound. Furthermore, an increasing quantity of evidence suggests that CD44 has various functions related to inflammatory disease. CD44 deficiency induces severe liver injury. CD44-hyaluronate mediates in lymphocyte T and monocyte adhesion to the endothelium, stimulates proinflammatory cytokine release from macrophages and participates in dedifferentiation phenotype of smooth muscle cells from contractile state to synthetic one. CD44 acts in atherosclerotic plaque destabilization and neointimal proliferation after percutaneous vascular interventions. CD44 participates in many stages of atherosclerosis. CD44 also is a cell membrane protein often overexpressed on tumor cells and, being both a cell-cell and cell-extracellular matrix adhesion protein, is well positioned to contribute to this process.

References

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